Noninvasive In Vivo Imaging and Monitoring of 3D-Printed Polycaprolactone Scaffolds Labeled with an NIR Region II Fluorescent Dye
Noninvasive In Vivo Imaging and Monitoring of 3D-Printed Polycaprolactone Scaffolds Labeled with an NIR Region II Fluorescent Dye
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DOI：
10.1021/acsabm.0c01587
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发表时间：
2021-03-08
影响因子：
4.7
通讯作者：
Huang, Dejian
中科院分区：
文献类型：
--
作者：
Jing, Linzhi;Sun, Mingtai;Huang, Dejian
利用超细纤维制备多孔支架用于组织再生已取得重大进展。然而，缺乏无创的体内跟踪方法使得无法在原位跟踪这些支架的命运。近红外区II （NIR-II, 1000-1700 nm）染料的发展为在体内进行深入组织穿透和高空间分辨率的无创可视化提供了可能。在此，我们开发了一种含有有机NIR-II小染料SY-1030和荧光标记大分子染料sy - co -PCL的聚己内酯（PCL）油墨，并通过电流体动力喷射（EHDJ）打印制备了高分辨率的NIR-II活性支架。所有打印支架在小鼠皮下植入后一周成像清晰。与含有SY-1030的支架相比，含有sy - co - pcl的支架发出的荧光强度可被跟踪长达三周。此外，可以通过调整染料浓度、激光功率和曝光时间来优化图像质量。这种NIR-II活性支架的优势体现在染料浓度较低、跟踪周期较长、体内稳定性较好。我们还在3个月的时间内证明了含有SY-COO-PCL的支架的生物相容性和生物降解性。所开发的NIR-II活性支架在生物聚合物植入物跟踪、组织重建监测和基于靶标位置的药物递送方面具有潜在的应用前景。
Significant progress has been made in fabricating porous scaffolds with ultrafine fibers for tissue regeneration. However, the lack of noninvasive tracking methods in vivo makes it impossible to track the fate of such scaffolds in situ. The development of near-infrared region II (NIR-II, 1000-1700 nm) dyes provides the possibility of performing noninvasive visualization with deep-tissue penetration and high spatial resolution in vivo. Herein, we developed a polycaprolactone (PCL) ink containing the small organic NIR-II dye SY-1030 and the fluorescently labeled macromolecular dye SY-COO-PCL and fabricated high-resolution NIR-II active scaffolds via electrohydrodynamic jet (EHDJ) printing. All printed scaffolds subcutaneously implanted in mice were clearly imaged one week after the operation. Compared with scaffolds containing SY-1030, the fluorescence intensity emitted from scaffolds containing SY-COO-PCL can be tracked for up to three weeks. Moreover, the image quality can be optimized by adjusting the dye concentration, laser power, and exposure time. The advantage of such NIR-II active scaffolds is evidenced by the lower dye concentration, longer tracking period, and better in vivo stability. We also demonstrated the biocompatibility and biodegradability of the scaffolds containing SY-COO-PCL over a 3-month period. The developed NIR-II active scaffolds have potential applications in biopolymer implant tracking, tissue reconstruction monitoring, and target-position-based drug delivery.