COMPARISON OF THE EFFECTS OF INSULIN AND H2O2 ON ADIPOCYTE GLUCOSE-TRANSPORT
COMPARISON OF THE EFFECTS OF INSULIN AND H2O2 ON ADIPOCYTE GLUCOSE-TRANSPORT
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DOI：
10.1002/jcp.1041100318
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
OLEFSKY, JM
中科院分区：
文献类型：
--
作者：
CIARALDI, TP;OLEFSKY, JM
研究了菊粉和胰岛素模拟物精胺和H2 O2刺激离体大鼠脂肪细胞中3-O-甲基葡萄糖转运的能力，以确定可能的共同作用机制。所有3种药物均引起葡萄糖转运初始速率的7- 12倍刺激，其中胰岛素是最有效的药物。胰岛素和精胺表现出类似的时间进程的开始，他们的刺激运输，最初的滞后之前，看到任何效果，然后逐渐上升，直到达到充分的效果。H2 O2激活葡萄糖转运的时间过程是不同的，因为刺激是在最早的时间点测试，然后逐渐上升到最大效果。胰蛋白酶消化细胞去除胰岛素受体，使细胞对胰岛素不敏感，但对精胺或H2 O2不敏感。然而，胰蛋白酶消化确实改变了过氧化氢作用的时程，导致初始滞后期出现和活化动力学的普遍减慢。用2，4-二硝基苯酚预处理细胞以降低ATP水平可阻止胰岛素和模拟物的刺激作用。所有3种试剂均显示出刺激葡萄糖转运的相似温度依赖性，导致线性Arrhenius图，活化能为10.2-11.4 kcal/mole。H_2O_2不直接作用于大鼠脂肪细胞的葡萄糖转运系统，胰岛素和H_2O最可能通过共同的能量依赖性生化途径来刺激葡萄糖转运，但H_2O_2进入胰岛素作用的初始步骤远端的刺激-反应序列。
The abilities of inuslin and the insulin mimickers spermine and H2O2 to stimulate 3-O-methyl glucose transport in isolated rat fat cells were studied to determine possible common mechanisms of action. All 3 agents caused a 7- to 12-fold stimulation of initial rates of glucose transport with insulin being the most effective agent. Insulin and spermine displayed similar time courses for the onset of their stimulation of transport; an initial lag before any effect was seen and then a gradual rise until the full effect was reached. The time course of H2O2 activation of glucose transport was different since stimulation was seen at the earliest time point tested and then gradually rose to the maximal effect. Trypsinization of cells removed insulin receptors and rendered the cells insensitive to insulin but not to spermine or H2O2. However, trypsinization did alter the time course of H2O2 action, causing an initial lag phase to appear and a general slowing of the activation kinetics. Pretreatment of cells with 2,4-dinitrophenol to lower ATP levels prevented the stimulatory effects of insulin and the mimickers. All 3 of the agents revealed a similar temperature dependency for stimulated glucose transport, resulting in linear Arrhenius plots with activation energies of 10.2-11.4 kcal/mole. H2O2 does not act directy on the glucose transport system of rat adipocytes and insulin and H2O most likey act through a common energy-dependent biochemical pathway to stimulate glucose transport, but H2O2 enters the stimulus-response sequence distal to the inital steps in insulin action.