DEVELOPMENTAL REGULATION OF T-CELL RECEPTOR GENE-EXPRESSION
DEVELOPMENTAL REGULATION OF T-CELL RECEPTOR GENE-EXPRESSION
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DOI：
10.1038/314103a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
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作者：
RAULET, DH;GARMAN, RD;TONEGAWA, S
与B细胞或其抗体产物相比，T淋巴细胞具有双重特异性，既可以诱导外来抗原，也可以对主要组织相容性复合体（MHC限制）中编码的细胞表面糖蛋白上的多态性决定因子1-4具有特异性。最近发现的t细胞受体糖蛋白5 - 7以及编码t细胞受体亚基的基因将有助于阐明MHC蛋白和外源抗原是由两个t细胞受体识别还是由单个受体识别。MHC限制的一个重要特征是，它似乎在很大程度上是由胸腺中表达的MHC抗原影响下的分化T细胞群获得的8 - 10，这表明前体T细胞是根据它们与宿主胸腺中表达的MHC决定因子的反应性选择的9 - 11。为了理解这一“胸腺教育”过程，了解t细胞受体基因表达的发育调控是必要的。在这里，我们报告了编码β-和γ-亚基的信使rna在未成熟胸腺细胞中相对丰富，而αmRNA的水平非常低。有趣的是，尽管α mrna水平在进一步发育过程中增加，而β mrna水平大致保持不变，但γmRNA在成熟T细胞中下降到非常低的水平，这表明γ基因在T细胞分化中起作用。
In contrast to B cells or their antibody products, T lymphocytes have a dual specificity, for both the eliciting foreign antigen and for polymorphic determinants on cell surface glycoproteins encoded in the major histocompatibility complex (MHC restriction)1–4. The recent identification of T-cell receptor glycoproteins5–7as well as the genes encoding T-cell receptor subunits will help to elucidate whether MHC proteins and foreign antigens are recognized by two T-cell receptors or by a single receptor. An important feature of MHC restriction is that it appears to be largely acquired by a differentiating T-cell population under the influence of MHC antigens expressed in the thymus8–10, suggesting that precursor T cells are selected on the basis of their reactivity with MHC determinants expressed in the host thymus9–11. To understand this process of ‘thymus education’, knowledge of the developmental regulation of T-cell receptor gene expression is necessary. Here we report that whereas messenger RNAs encoding theβ-andγ-subunits are relatively abundant in immature thymocytes,αmRNA levels are very low. Interestingly, whereasαmRNA levels increase during further development andβmRNA levels stay roughly constant,γmRNA falls to very low levels in mature T cells, suggesting a role for theγgene in T-cell differentiation.