Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene.
Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene.
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大末端重复的亚片段引起小鼠乳腺肿瘤病毒和邻近基因的糖皮质激素反应性表达。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
Bernd Groner
中科院分区：
文献类型：
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作者：
N. Hynes;A. V. Ooyen;N. Kennedy;Peter Herrlich;H. Ponta;Bernd Groner
将小鼠乳腺肿瘤病毒 (MMTV) 前病毒 DNA 转染至培养细胞后，DNA 以糖皮质激素敏感的方式转录。 MMTV 的大末端重复 (LTR) 区域长 1,328 个核苷酸，包含激素反应所需的调节信息。我们构建了MMTV LTR-胸苷激酶(tk)嵌合基因，并测试了LTK-APRT-小鼠细胞转化后LTR中含有各种缺失的分子的生物活性。在 TK+ 转化体中，LTR-tk 嵌合 RNA 和真实的 tk RNA 均被正确启动和转录。嵌合RNA以及tk RNA的合成受激素调节。 RNA起始位点5'端含有202个LTR DNA核苷酸的质粒对糖皮质激素完全敏感； 50个核苷酸仍然会产生残余诱导性。
After transfection of mouse mammary tumor virus (MMTV) proviral DNA into cultured cells, the DNA is transcribed in a glucocorticoid-sensitive fashion. The large terminal repeat (LTR) region of MMTV is 1,328 nucleotides long and contains the regulatory information necessary for the hormonal response. We have constructed a MMTV LTR-thymidine kinase (tk) chimeric gene and have tested the biological activity of molecules containing various deletions in the LTR after transformation of LTK- APRT- mouse cells. In the TK+ transformants, both a LTR- tk chimeric RNA and an authentic tk RNA are correctly initiated and transcribed. The synthesis of the chimeric RNA as well as that of the tk RNA is hormonally regulated. A plasmid containing 202 nucleotides of LTR DNA 5' to the RNA initiation site is fully sensitive to glucocorticoids; 50 nucleotides still cause a residual inducibility.