Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus
复制标题
DOI：
10.1128/jcm.00847-13
复制
发表时间：
2013-08-01
影响因子：
9.4
通讯作者：
Toeroek, M. Estee
中科院分区：
文献类型：
--
作者：
Cartwright, Edward J. P.;Paterson, Gavin K.;Toeroek, M. Estee
mecC 耐甲氧西林金黄色葡萄球菌 (MRSA) 的出现给临床微生物实验室带来了诊断挑战。使用 Vitek 2 系统，我们测试了一组 896 个金黄色葡萄球菌分离株，发现苯唑西林敏感/头孢西丁耐药谱对于 mecC MRSA 分离株的鉴定灵敏度为 88.7%，特异性为 99.5%。通过细菌全基因组测序确定的 mecC 基因的存在被用作金标准。该谱可以提供一种零成本筛选方法来鉴定 mecC 阳性 MRSA 菌株。
The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sensitivity of 88.7% and a specificity of 99.5% for the identification of mecC MRSA isolates. The presence of the mecC gene, determined by bacterial whole-genome sequencing, was used as the gold standard. This profile could provide a zero-cost screening method for identification of mecC-positive MRSA strains.