The mRNA-binding ribosomal protein S26 as a molecular marker in plants:: molecular cloning, sequencing and differential gene expression during environmental stress
The mRNA-binding ribosomal protein S26 as a molecular marker in plants:: molecular cloning, sequencing and differential gene expression during environmental stress
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DOI：
10.1016/s0167-4781(99)00050-0
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发表时间：
1999-06-09
期刊：
影响因子：
--
通讯作者：
Strid, Å
中科院分区：
文献类型：
--
作者：
Brosché, M;Strid, Å
使用基因作为环境胁迫的转录标记的一个条件是其特异性和差异表达。为了用作这样的标记，从豌豆（Pisum sativum L.）被克隆并完全测序该基因（PsRPS 26）被证明是差异调节臭氧和UV-B辐射以相反的方式。臭氧导致mRNA水平增加，而W-B导致S26转录丰度降低。因此，PsRPS 26的表达可用作区分这两种环境胁迫的分子标记。(C)1999 Elsevier Science B. V.保留所有权利。
One condition for using a gene as a transcriptional marker for environmental stress is its specific and differential expression. In order to be used as such a marker, the ribosomal protein S26 cDNA from pea (Pisum sativum L.) was cloned and fully sequenced. The gene (PsRPS26) was shown to be differentially regulated by ozone and UV-B radiation in opposite ways. Ozone gave rise to increased mRNA levels, whereas W-B led to a decrease in S26 transcript abundance. Thus, the expression of PsRPS26 can be used as a molecular marker to differentiate between these two environmental stresses. (C) 1999 Elsevier Science B.V. All rights reserved.