Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress.
Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress.
复制标题
两个高羊茅 (Festuca arundinacea) 品种对铅 (Pb) 胁迫反应的转录组分析
DOI：
10.1186/s12864-016-3479-3
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发表时间：
2017-02-10
期刊：
影响因子：
4.4
通讯作者：
Fu J
中科院分区：
文献类型：
--
作者：
Li H;Hu T;Amombo E;Fu J
背景铅（Pb）是毒性最大的重金属环境污染物之一。高羊茅是一种重要的冷季型草坪草，能耐受和积累大量的铅。为了估计与铅响应相关的基因和与铅耐受性和积累相关的分子机制，我们分析了高羊茅的转录组响应于Pb treatment. ResultsRNA测序在两个高羊茅品种，耐铅西尔维拉多和铅敏感的AST 7001。从高羊茅叶中获得了代表25，415个单基因的总共810，146个组装的独特转录物。其中，3,696差异表达基因（DEG）之间的铅处理（1000 mg/L）和未处理的样品检测。基因本体（GO）和途径富集分析表明，DEG主要参与能量代谢、萜类化合物和聚酮化合物代谢以及碳水化合物代谢相关途径。随机选取的16个基因的表达模式与Solexa定量逆转录PCR分析结果一致。此外，与两个品种对铅胁迫的共同转录响应相比，玉米素合成、柠檬烯和蒎烯降解、吞噬体等多个基因的调控是一个品种独有的.ConclusionsThe tall fescue assembled transcriptome为草坪草对重金属胁迫响应的基因组学研究提供了重要的分子资源.这可能是两个高羊茅品种铅耐性或铅积累的特异性单基因表达差异的原因。本研究为探讨高羊茅及其它相关草坪草对铅的耐性和积累的分子基础提供了新的思路。
BackgroundLead (Pb) is one of the most toxic heavy metal environmental pollutants. Tall fescue is an important cold season turf grass which can tolerate and accumulate substantial amount of Pb. To estimate genes related to Pb response and the molecular mechanism associated with Pb tolerance and accumulation, we analyzed the transcriptome of tall fescue in response to Pb treatment.ResultsRNA-sequencing was performed in two tall fescue cultivars, Pb tolerant Silverado and Pb sensitive AST7001. A total of 810,146 assembled unique transcripts representing 25,415 unigenes were obtained from the tall fescue leaves. Among the panel, 3,696 differentially expressed genes (DEGs) were detected between the Pb treated (1000 mg/L) and untreated samples. Gene ontology (GO) and pathway enrichment analysis demonstrated that the DEGs were mainly implicated in energy metabolism, metabolism of terpenoids and polyketides, and carbohydrate metabolism related pathways. The expression patterns of 16 randomly selected genes were in consistent with that from the Solexa analysis using quantitative reverse-transcription PCR. In addition, compared to the common transcriptional response to Pb stress in both cultivars, the regulation of numerous genes including those involved in zeatin biosynthesis, limonene and pinene degradation, phagosome was exclusive to one cultivar.ConclusionsThe tall fescue assembled transcriptome provided substantial molecular resources for further genomics analysis of turfgrass in response to heavy metal stress. The significant expression difference of specific unigenes may account for Pb tolerance or accumulation in two different tall fescue cultivars. This study provided new insights for the investigation of the molecular basis of Pb tolerance and accumulation in tall fescue as well as other related turf grass species.