The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis
The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis
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DOI：
10.1093/emboj/18.4.916
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发表时间：
1999-02-15
期刊：
影响因子：
11.4
通讯作者：
Dyson, N
中科院分区：
文献类型：
--
作者：
Du, W;Dyson, N
在果蝇胚胎发生的第17个细胞周期中，G（1）的首次出现伴随着E2F依赖性转录的下调。产生并分析rbf的突变等位基因以确定RBF在该过程中的作用。缺乏母体和合子RBF产物的胚胎显示PCNA和RNR2的组成性表达，这两个E2F调节基因，表明RBF是其转录抑制所必需的。尽管E2F靶基因普遍表达，但大多数表皮细胞正常进入G（1）。这些细胞中的许多进入异位S期，而不是在G（1）中暂停，直到细胞周期进展的适当时间。这些结果表明，RBF对E2F靶基因的抑制对于维持G（1）期是必需的，而不是启动G（1）期。RBF缺陷胚胎的表型表明，在果蝇胚胎发生过程中，RBF具有与dacapo和fizzy相关的G（1）导入作用互补的功能。
The first appearance of G(1) during Drosophila embryogenesis, at cell cycle 17, is accompanied by the downregulation of E2F-dependent transcription. Mutant alleles of rbf were generated and analyzed to determine the role of RBF in this process. Embryos lacking both maternal and zygotic RBF products show constitutive expression of PCNA and RNR2, two E2F-regulated genes, indicating that RBF is required for their transcriptional repression, Despite the ubiquitous expression of E2F target genes, most epidermal cells enter G(1) normally. Rather than pausing in G(1) until the appropriate time For cell cycle progression, many of these cells enter an ectopic S-phase. These results indicate that the repression of E2F target genes by RBF is necessary for the maintenance but not the initiation of a G(1) phase. The phenotype of RBF-deficient embryos suggests that rbf has a function that is complementary to the roles of dacapo and fizzy-related in the introduction of G(1) during Drosophila embryogenesis.