C-reactive protein and serum amyloid a mrna stability following induction by cytokines
C-reactive protein and serum amyloid a mrna stability following induction by cytokines
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DOI：
10.1006/cyto.1996.0072
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发表时间：
1996-07-01
期刊：
影响因子：
3.8
通讯作者：
Kushner, I
中科院分区：
文献类型：
--
作者：
Lozanski, G;Jiang, SL;Kushner, I
我们确定了细胞因子撤药对C-反应蛋白（CRP）和血清淀粉样蛋白A（SAA）mRNA丰度的影响，Hep 3B细胞用白细胞介素6加白细胞介素1 β预诱导24小时后。在细胞因子撤除后，CRP转录率迅速下降至不可检测的水平，mRNA水平下降，半消失时间约为2.5 h。鉴于此时发生的CRP转录量相对较少，该值可能密切反映了CRP mRNA的实际半衰期，相反，大量SAA转录持续至少8 h，而SAA mRNA则下降，半消失时间约为8.5h，在这些条件下，不可能确定SAA mRNA的半衰期，但显然不大于8.5h。放线菌素D（ActD）和放线菌酮均增强SAA mRNA的稳定性，强烈表明SAA mRNA降解需要合成短寿命蛋白，ActD也增强了CRP mRNA稳定性，但放线菌酮没有持久的稳定作用，表明复杂的调节过程。这些研究提供了对用[IL-6 + IL-1 β]诱导后CRP和SAA mRNA的稳定性以及调节其降解的机制的深入了解。(C)1996年学术出版社
We determined the effects of cytokine withdrawal on C-reactive protein (CRP) and serum amyloid A (SAA) mRNA abundance in Hep3B cells following 24 h of preinduction with interleukin 6 plus interleukin 1 beta. After cytokine withdrawal, CRP transcription rate rapidly fell to undetectable levels and mRNA levels fell with a half-disappearance time of about 2.5 h, In view of the relatively small amount of CRP transcription occurring at this time, it is likely that this value closely reflects the actual half-life of CRP mRNA, In contrast, substantial SAA transcription continued for at least 8 h, while SAA mRNA fell with a half-disappearance time of about 8.5 h, It is not possible, under these conditions, to determine SAA mRNA half-life, but it clearly was no greater than 8.5 h, Both Actinomycin D (ActD) and cycloheximide enhanced the stability of SAA mRNA, strongly suggesting that SAA mRNA degradation requires synthesis of a short-lived protein, CRP mRNA stability was also enhanced by ActD, but cycloheximide did not have a protracted stabilizing effect, suggesting complex regulatory processes. These studies provide insight into the stability of CRP and SAA mRNA following induction with [IL-6 + IL-1 beta] and into the mechanisms regulating their degradation. (C) 1996 Academic Press Limited