Genome-wide location and function of DNA binding proteins
Genome-wide location and function of DNA binding proteins
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DOI：
10.1126/science.290.5500.2306
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发表时间：
2000-12-22
期刊：
影响因子：
56.9
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Ren, B;Robert, F;Young, RA
了解DNA结合蛋白如何控制全局基因表达和染色体维持需要了解这些蛋白在体内发挥功能的染色体位置。我们开发了一种微阵列方法，揭示了DNA结合蛋白质的全基因组定位，并使用这种方法来监测酵母中基因特异性转录激活因子的结合。位置和表达谱的组合用于鉴定其表达直接由Gal4和Ste12控制的基因，因为细胞分别响应于碳源和交配信息素的变化。结果确定了由两种激活剂中的每一种协调调节的途径，并揭示了Gal4和Ste12先前未知的功能。全基因组定位分析将有助于研究基因调控网络、基因功能和基因组维护。
Understanding how DNA binding proteins control global gene expression and chromosomal maintenance requires knowledge of the chromosomal Locations at which these proteins function in vivo. We developed a microarray method that reveals the genome-wide Location of DNA-bound proteins and used this method to monitor binding of gene-specific transcription activators in yeast. A combination of location and expression profiles was used to identify genes whose expression is directly controlled by Gal4 and Ste12 as cells respond to changes in carbon source and mating pheromone, respectively. The results identify pathways that are coordinately regulated by each of the two activators and reveal previously unknown functions for Gal4 and Ste12. Genome-wide Location analysis will facilitate investigation of gene regulatory networks, gene function, and genome maintenance.