Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
复制标题
DOI：
10.1016/s0015-0282(02)04240-1
复制
发表时间：
2002-12-01
影响因子：
6.7
通讯作者：
González, E
中科院分区：
文献类型：
--
作者：
Novaro, V;Pustovrh, C;González, E
目的：评估胚胎源性一氧化氮（NO）启动的相互信号相互作用，通过增加子宫基质中明胶酶A（基质金属蛋白酶2，MMP 2）的产生来促进着床。设计：实验动物研究。设置：生殖生理学研究实验室。动物：14周龄的雌性同系Wistar大鼠。干预：阴道涂片以确认妊娠。输卵管结扎以避免胚泡下降到子宫腔。血浆渗出测定法定位子宫胚泡着床部位。用NO供体和一氧化氮合酶（NOS）抑制剂处理器官培养物.主要结果测量：MMP 2和NO的表达通过组织提取物的蛋白质印迹和酶谱以及组织切片的免疫荧光进行评估.结果：在早孕大鼠的子宫提取物中发现MMP 2活性增加，并集中在着床部位。免疫定位实验表明，诱导型NOS表达的着床胚泡表面上的子宫上皮细胞在MMP 2表达增加的网站。在器官培养实验中，NO供体被发现增加，而NOS抑制剂被发现降低MMP 2活性在子宫组织sections.Conclusions（S）：囊胚衍生的NO有助于子宫衍生的MMP 2，一个重要组成部分的生产植入和启动胎盘。
Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma.Design: Experimental animal studies.Setting: Reproductive-physiology research laboratory.Animal(s): Female syngeneic Wistar rats aged 14 weeks.Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors.Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections.Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections.Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation.