Construction of an integrated genetic map based on maternal and paternal lineages of tea (Camellia sinensis)
Construction of an integrated genetic map based on maternal and paternal lineages of tea (Camellia sinensis)
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DOI：
10.1007/s10681-013-0908-0
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发表时间：
2013-05-01
期刊：
影响因子：
1.9
通讯作者：
Lin, Shun-Fu
中科院分区：
文献类型：
--
作者：
Hu, Chih-Yi;Lee, Tair-Chyang;Lin, Shun-Fu
茶树自交不亲和性的存在，使茶树重要性状的遗传研究困难重重。此外，一个新品种的开发通常需要20年以上，因为从幼苗到成熟植株需要多年的性状调查。遗传图谱是遗传研究和育种的重要工具。在这项研究中，我们已经开发了一个完整的遗传图谱的茶树（茶树）分离F1群体来自两个商业品种（'TTES 19'和'TTES 8'）之间的杂交。共筛选出574个多态性标记（包括SSR、CAPS、STS、AFLP、ISSR和RAPD），其中69个标记的分离偏度极显著（P < 0.001）（12.0%）。在505个标记中，父本标记265个（52.5%），母本标记163个（32.3%），双杂合显性标记65个（12.9%），共显性标记12个（2.4%）。共显性标记和双杂合显性标记被用作父本和母本图谱整合的桥梁位点。结果表明，该图谱共包含367个连锁标记，其中SSR标记36个，CAPS标记3个，STS标记1个，AFLP标记250个，ISSR标记13个，RAPD标记64个，分属于18个连锁群。连锁群的总长度为4482.9cM，图位密度为12.2cM。该遗传图谱是目前遗传覆盖率最高的遗传图谱，可用于比较作图、QTL定位和标记辅助选择。
Genetic study on important traits of tea is difficult because of its self-incompatibility in nature. Moreover, development of a new variety usually needs more than 20 years, since it takes many years from seedling to matured plants for trait investigation. Genetic map is an essential tool for genetic study and breeding. In this study, we have developed an integrated genetic map of tea (Camellia sinensis) using a segregating F1 population derived from a cross between two commercial cultivars ('TTES 19' and 'TTES 8'). A total of 574 polymorphic markers (including SSR, CAPS, STS, AFLP, ISSR and RAPD), 69 markers with highly significant levels of segregation distortion (P < 0.001) (12.0 %) were excluded from further analyses. Of the 505 mapped markers, there were 265 paternal markers (52.5 %), 163 maternal markers (32.3 %), 65 doubly heterozygous dominant markers (12.9 %), and 12 co-dominant markers (2.4 %). The co-dominant markers and doubly heterozygous dominant markers were used as bridge loci for the integration of the paternal and maternal maps. The integrated map comprised 367 linked markers, including 36 SSR, 3 CAPS, 1 STS, 250 AFLP, 13 ISSR and 64 RAPD that were assigned to 18 linkage groups. The linkage groups represented a total map length of 4482.9 cM with a map density of 12.2 cM. This genetic map has the highest genetic coverage so far, which could be applied to comparative mapping, QTL mapping and marker assisted selection in the future.