ISOLATION OF A TRANSFORMING SEQUENCE FROM A HUMAN BLADDER-CARCINOMA CELL-LINE
ISOLATION OF A TRANSFORMING SEQUENCE FROM A HUMAN BLADDER-CARCINOMA CELL-LINE
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DOI：
10.1016/0092-8674(82)90100-3
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
WEINBERG, RA
中科院分区：
文献类型：
--
作者：
SHIH, C;WEINBERG, RA
分离出能够转化小鼠成纤维细胞的人膀胱癌细胞 DNA 成分。致癌序列最初是从噬菌体 lambda 基因组文库中分离出来的，该文库由携带人类致癌基因的转染小鼠细胞的 DNA 制成。在质粒载体 pBR322 中扩增了具有转化活性的 6.6 kb [千碱基] 亚克隆插入片段。亚克隆的癌基因被用作Southern印迹分析中的序列探针。癌基因似乎源自正常细胞 DNA 中的序列。迄今为止，结构分析未能揭示癌基因与其正常细胞同源物之间的差异。该癌基因与在源自结肠癌、肺癌和神经母细胞瘤的多种其他类型人类肿瘤细胞系[包括SW-2-2细胞、Lx1-2细胞、A549-1细胞、EJ-6-2细胞、SH-1-1细胞、T24-8-5细胞和HL60-1-6细胞]中检测到的转化序列无关。 EJ 膀胱癌基因似乎与人 T24 膀胱癌细胞系中活跃的一种癌基因密切相关。在几种膀胱癌细胞系中，癌基因似乎几乎没有发生扩增（如果有的话）。
The component of human bladder carcinoma cell DNA that is able to transform mouse fibroblasts was isolated. The oncogenic sequence was isolated initially from a phage lambda genomic library made from DNA of a transfected mouse cell carrying the human oncogene. A subcloned insert of 6.6 kb [kilobases] that carried transforming activity was amplified in the plasmid vector pBR322. The subcloned oncogene was used as a sequence probe in Southern blot analyses. The oncogene appears to derive from sequences present in normal cellular DNA. Structural analysis has failed so far to reveal differences between the oncogene and its normal cellular homolog. The oncogene is unrelated to transforming sequences detected in a variety of other types of human tumor cell lines [including SW-2-2 cells, Lx1-2 cells, A549-1 cells, EJ-6-2 cells, SH-1-1 cells, T24-8-5 cells and HL60-1-6 cells] derived from colonic and lung carcinoma and from neuroblastoma. The EJ bladder oncogene appears closely related to one that in active in the human T24 bladder carcinoma cell line. The oncogene appears to have undergone little, if any, amplification in several bladder carcinoma cell lines.