Effect of oral iron supplementation on oxidative stress and colonic inflammation in rats with induced colitis
Effect of oral iron supplementation on oxidative stress and colonic inflammation in rats with induced colitis
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DOI：
10.1046/j.1365-2036.2001.01113.x
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发表时间：
2001-12-01
影响因子：
7.6
通讯作者：
Allard, JP
中科院分区：
文献类型：
--
作者：
Carrier, J;Aghdassi, E;Allard, JP
背景：补铁可能会增加溃疡性结肠炎的疾病活动性，可能是通过芬顿反应产生活性氧而增加的。目的：评估两种剂量的口服铁剂对实验性结肠炎肠道炎症和氧化应激的影响。方法：通过饮用水中添加 5% 右旋糖酐硫酸钠 7 天来诱导大鼠结肠炎。首先，使用2×2因子设计，给予或不给予葡聚糖硫酸钠的大鼠接受常规饮食或含铁3%/kg饮食的饮食。其次，对右旋糖酐硫酸钠诱导的结肠炎大鼠添加0.3%/kg的铁饮食，并与右旋糖酐硫酸钠和常规饮食的大鼠进行比较。测量体重变化、组织学评分、结肠长度、直肠出血、血浆和结肠脂质过氧化物、结肠谷胱甘肽过氧化物酶以及血浆维生素E和C。还对粪便中的血红素、总铁、游离铁和乙二胺四乙酸螯合铁进行了分析。结果：3% 铁和 0.3% 铁增加了葡聚糖硫酸钠诱导的结肠炎的活性，表现为较高的组织学评分、较重的直肠出血和结肠进一步缩短。这与脂质过氧化增加和抗氧化维生素减少有关。可用于芬顿反应的粪便铁以剂量依赖性方式增加。结论：口服铁补充剂增强了葡聚糖硫酸钠诱导的结肠炎的活性，并且与氧化应激的增加有关。
Background: Iron supplementation may increase disease activity in ulcerative colitis, possibly through the production of reactive oxygen species from the Fenton reaction.Aim: To assess the effects of two doses of oral iron on intestinal inflammation and oxidative stress in experimental colitis.Methods: Colitis was induced in rats by giving 5% dextran sulphate sodium in drinking water for 7 days. First, using a 2 x 2 factorial design, rats with or without dextran sulphate sodium received the regular diet or a diet containing iron 3%/kg diet. Second, rats with dextran sulphate sodium-induced colitis were supplemented with iron 0.3%/kg diet and compared with rats on dextran sulphate sodium and regular diet. The body weight change, histological scores, colon length, rectal bleeding, plasma and colonic lipid peroxides, colonic glutathione peroxidase and plasma vitamin E and C were measured. Faecal analysis for haem and total, free and ethylenediaminetetra-acetic acid-chelatable iron was also performed.Results: Iron 3% and iron 0.3% increased the activity of dextran sulphate sodium-induced colitis, as demonstrated by higher histological scores, heavier rectal bleeding and further shortening of the colon. This was associated with increased lipid peroxidation and decreased antioxidant vitamins. Faecal iron available to the Fenton reaction was increased in a dose-dependent manner.Conclusions: Iron supplementation taken orally enhanced the activity of dextran sulphate sodium-induced colitis and is associated with an increase in oxidative stress.