Mesotheliomas in rats following inoculation with asbestos.
Mesotheliomas in rats following inoculation with asbestos.
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接种石棉后，大鼠的间皮瘤。
DOI：
10.1038/bjc.1969.70
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发表时间：
1969-09
影响因子：
8.8
通讯作者：
Berry, G
中科院分区：
文献类型：
--
作者：
Wagner, J C;Berry, G
方法用乙醚麻醉大鼠，然后将一根连接到双向水龙头的针插入右腋窝第二乳头的水平。双向水龙头的一个臂连接到毛细管压力计，当针到达胸膜腔时，毛细管压力计给出负读数。结核菌素注射器含有0.5毫升。首先用超声波然后用手充分摇动的悬浮液被附着到水龙头的臂上，并将材料引入胸膜腔。观察到0.1毫升。保留在针头和水龙头中，因此大鼠实际上接受了 0.4 毫升。 50毫克/毫升的悬浮液。因此接受了 20 毫克。灰尘。青石棉
METHODSThe rats were anaesthetised with ether and a needle attached to a two-way tap was then introduced into the right axilla at the level of the second nipple. One arm of the two-way tap was attached to a capillary manometer, which gave a negative reading when the needle reached the pleuralcavity. A tuberculin syringe containing 0 5 ml. of the suspension, which had been well shaken, first ultrasonically and then by hand, was attached to the arm of the tap and the material introduced into the pleural cavity. It was observed that 0.1 ml. remained in the needle and tap, so that the rat actually received 0.4 ml. of a suspension of 50 mg./ml. and hence received 20 mg. of dust. The crocidolite