COMPARISON OF THE 3,5-DINITROSALICYLIC ACID AND NELSON-SOMOGYI METHODS OF ASSAYING FOR REDUCING SUGARS AND DETERMINING CELLULASE ACTIVITY
COMPARISON OF THE 3,5-DINITROSALICYLIC ACID AND NELSON-SOMOGYI METHODS OF ASSAYING FOR REDUCING SUGARS AND DETERMINING CELLULASE ACTIVITY
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DOI：
10.1016/0141-0229(85)90111-5
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
SADDLER, JN
中科院分区：
文献类型：
--
作者：
BREUIL, C;SADDLER, JN
比较了3，5-二硝基水杨酸（DNS）法和碱性铜法测定哈茨木霉（Trichoderma harzianum）E58菌株纤维素酶解过程中还原糖的含量。DNS方法更容易受到孵育条件和源自木质纤维素基质的组分的影响。内切-1，4-β- D-葡聚糖酶[1，4-（1，3; 1，4）-β-用DNS测定法获得的D-葡聚糖4-葡聚糖水解酶，EC 3.2.1.4]值总是显著高于用碱性Cu法获得的值，并且没有给出与半缩醛还原基团的实际数目成比例的还原值。碱性Cu测定不受底物的聚合度的影响。尽管后一种方法似乎上级于DNS测定法，但其仍受孵育条件、底物性质和其他纤维素组分对每种特定酶测定法的影响的影响。
A comparison was made between the 3,5-dinitrosalicylic acid (DNS) and alkaline Cu methods of assaying for reducing sugars released during the enzymatic hydrolysis of cellulose by culture filtrates from Trichoderma harzianum E58. The DNS method is more readily influenced by the incubation conditions and by components derived from lignocellulosic substrates. The endo-1,4-.beta.-D-glucanase [1,4-(1,3;1,4)-.beta.-D-glucan 4-glucanohydrolase, EC 3.2.1.4]values obtained with the DNS assay were always considerably higher than those obtained with the alkaline Cu method and did not give reducing values that were proportional to the actual number of hemiacetal reducing groups. The alkaline Cu assay was not affected by the degree of polymerixation of the substrate. Although this latter method appeared to be superior to the DNS assay it was still affected by the incubation conditions, nature of the substrate and the influence of other celluluse components on each of the specific enzyme assays.