Taxonomic evaluation of Streptomyces albus and related species using multilocus sequence analysis and proposals to emend the description of Streptomyces albus and describe Streptomyces pathocidini sp nov.
Taxonomic evaluation of Streptomyces albus and related species using multilocus sequence analysis and proposals to emend the description of Streptomyces albus and describe Streptomyces pathocidini sp nov.
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DOI：
10.1099/ijs.0.058107-0
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发表时间：
2014-03-01
影响因子：
2.8
通讯作者：
Metcalf, W. W.
中科院分区：
文献类型：
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作者：
Labeda, D. P.;Doroghazi, J. R.;Metcalf, W. W.
利用16 S rRNA基因序列对链霉菌属进行系统发育分析，白色链霉菌亚种（Streptomyces albus subsp.）albus NRRL B-1811（T）与具有相同或几乎相同的16 S rRNA基因序列的五个其它物种形成一个簇。此外，这些其他物种，包括链霉菌almquistii NRRL B-1685（T）、絮状链霉菌NRRL B-2465（T）、吉氏链霉菌NRRL B-1335（T）和兰贡链霉菌NRRL B-12378（T）的形态和生理特征非常相似。这一簇具有特别的分类学意义，因为白色链霉菌是链霉菌属的模式种。利用管家基因atpD、gyrB、recA、rpoB和trpB的部分序列对相关菌株进行多位点序列分析（MLSA），并确认先前报道的表型特征。这五个菌株形成了一个连贯的集群支持的100%的自举值在系统发育树中产生的序列比对制备的管家基因的序列连接，并观察到相同的树拓扑结构，使用各种不同的树制作算法。除一株S.绒球菌NRRL B-2465（T）的所有5个管家基因座的序列都相同，但NRRL B-2465（T）与其他菌株的MLSA进化距离仍为0.005，该值低于为物种水平相关性提出的0.007 MLSA进化距离阈值。这些数据支持重新分类S的建议。almquistii，S. flocculus、S. gibsonii和S. rangoonensis为S. albus，以NRRL B-1811（T）为模式菌株。MLSA序列数据库还证明了快速和最终确认ARS培养物保藏中心内的许多菌株先前被错误鉴定为S的亚种的实用性。白色链霉菌（Streptomyces albus subsp. pathocidicus应该重新描述为一个新的物种，即致病杀链霉菌新种，模式菌株NRRL B-24287（T）。
In phylogenetic analyses of the genus Streptomyces using 16S rRNA gene sequences, Streptomyces albus subsp. albus NRRL B-1811(T) forms a cluster with five other species having identical or nearly identical 16S rRNA gene sequences. Moreover, the morphological and physiological characteristics of these other species, including Streptomyces almquistii NRRL B-1685(T), Streptomyces flocculus NRRL B-2465(T), Streptomyces gibsonii NRRL B-1335(T) and Streptomyces rangoonensis NRRL B-12378(T) are quite similar. This cluster is of particular taxonomic interest because Streptomyces albus is the type species of the genus Streptomyces. The related strains were subjected to multilocus sequence analysis (MLSA) utilizing partial sequences of the housekeeping genes atpD, gyrB, recA, rpoB and trpB and confirmation of previously reported phenotypic characteristics. The five strains formed a coherent cluster supported by a 100% bootstrap value in phylogenetic trees generated from sequence alignments prepared by concatenating the sequences of the housekeeping genes, and identical tree topology was observed using various different tree-making algorithms. Moreover, all but one strain, S. flocculus NRRL B-2465(T), exhibited identical sequences for all of the five housekeeping gene loci sequenced, but NRRL B-2465(T) still exhibited an MLSA evolutionary distance of 0.005 from the other strains, a value that is lower than the 0.007 MLSA evolutionary distance threshold proposed for species-level relatedness. These data support a proposal to reclassify S. almquistii, S. flocculus, S. gibsonii and S. rangoonensis as later heterotypic synonyms of S. albus with NRRL B-1811(T) as the type strain. The MLSA sequence database also demonstrated utility for quickly and conclusively confirming that numerous strains within the ARS Culture Collection had been previously misidentified as subspecies of S. albus and that Streptomyces albus subsp. pathocidicus should be redescribed as a novel species, Streptomyces pathocidini sp. nov., with the type strain NRRL B-24287(T).