CELL PROLIFERATION IN EMBRYONIC MOUSE NEURAL TUBE FOLLOWING URETHANE EXPOSURE
CELL PROLIFERATION IN EMBRYONIC MOUSE NEURAL TUBE FOLLOWING URETHANE EXPOSURE
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DOI：
10.1016/0012-1606(69)90010-4
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发表时间：
1969-01-01
影响因子：
2.7
通讯作者：
KAUFFMAN, SL
中科院分区：
文献类型：
--
作者：
KAUFFMAN, SL
正常10天小鼠胚胎和暴露于氨基甲酸乙酯的胚胎的细胞周期的比较放射自显影研究显示，实验组的周期时间增加了4.5小时。最初尿烷干扰一些未标记的有丝分裂前核迁移到胚泡区，导致室外有丝分裂。有丝分裂指数和中期比例的一过性增加表明有丝分裂时间延长，但持续时间较短，不会显著增加总体周期时间。在尿烷存在下掺入胸腺嘧啶核苷-3H的细胞的G2期显著延长，S期和G1期也显著延长，一些受损细胞在分裂间期或有丝分裂中死亡。这表明周期短的细胞可能会优先被尿烷杀死，从而将平均周期时间的确定留给更分化的细胞。
A comparative autoradiographic study of the cell cycle in normal 10-day mouse embryos and in embryos exposed to urethane showed an increase of 4.5 hours in the cycle time of the experimental group. Initially urethane interfered with the migration of some unlabeled premitotic nuclei to the germinal zone, resulting in extraventricular mitoses. A transient increase in the mitotic index and proportion of metaphases suggested a lengthened mitotic time but this was of brief duration and would not contribute significantly to the overall increased cycle time. The G2period of cells which incorporated thymidine-3H in the presence of urethane was significantly lengthened as were the S and G1periods and some injured cells died in interphase or mitosis.The new lengthened generation time of embryo neural tubes exposed to urethane resembles that of older, 11-day embryos. This suggests the possibility that cells with a short cycle may be preferentially killed by urethane, leaving the determination of mean cycle time to more differentiated cells.