Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes
Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes
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DOI：
10.1016/j.jff.2017.06.051
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发表时间：
2017-09-01
影响因子：
5.6
通讯作者：
Li, Chao
中科院分区：
文献类型：
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作者：
Ji, Desheng;You, Lijun;Li, Chao
采用DEAE-Sepharose快速流动柱对羊栖菜多糖进行纯化，得到羊栖菜多糖组分（SFP-P1）。正如预期的那样，SFP-P1主要含有碳水化合物（87%）和不到1%的蛋白质。在碳水化合物中，它含有约10%的硫酸盐和18%的糖醛酸。SFP-P1由L-岩藻糖（13.17%）、半乳糖（4.28%）、葡萄糖（1.95%）、木糖（5.50%）和甘露糖（75.10%）组成，平均分子量为113 kDa。甘露糖和L-岩藻糖是两种主要的单糖。此外，（1）-或（1 -> 6）-连接、（1 -> 2）-或（1 -> 4）-连接和（1 - 3）-连接的糖基键分别占分子中所有糖基键的1.62%、60.69%和37.69%。SFP-P1的糖苷键类型为-> 3，6）-alpha-D-Manp（1 4）-alpha-D-GalAp（1 ->，-> 4）-beta-D-Xylp（1 ->和3，4）-alpha-D-GlcAp（1）。SFP-P1还抑制UVB诱导的MMP-1和MMP-9的表达。(C)2017爱思唯尔有限公司出版
In this study, Sargassum fusiforme polysaccharide was purified using a DEAE-Sepharose fast-flow column to obtain Sargassum fusiforme polysaccharides fraction (SFP-P1). As expected, the SFP-P1 contains mainly carbohydrates (87%) with less than 1% protein. Within the carbohydrate, it contains about 10% sulfate and 18% uronic acids. SFP-P1 consisted of L-fucose (13.17%), galactose (4.28%), glucose (1.95%), xylose (5.50%), and mannose (75.10%) with an average molecular weight of 113 kDa. Mannose and L-fucose were two predominant monosaccharides. In addition, (1)-linked or (1 -> 6) -linked, (1 -> 2) -linked or (1 -> 4) linked and (1 3) -linked glycosyl linkages accounted for 1.62%, 60.69% and 37.69% of all linkages in the molecule, respectively. The glycosidic linkage types of SFP-P1 were proven to be -> 3,6)-alpha-D-Manp (1 4)-alpha-D-GalAp(1 ->, -> 4)-beta-D-Xylp(1 -> and 3,4)-alpha-D-GIcAp(1 The results indicated the SFP-P1 had cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through stimulation of SOD, GSH-P-x enzymes activities and ROS. Also SFP-P1 suppressed the UVB-induced expression of MMP-1 & MMP-9. (C) 2017 Published by Elsevier Ltd.