The transfer of sulfate among phenolic compounds with 3',5'-diphosphoadenosine as coenzyme.
The transfer of sulfate among phenolic compounds with 3',5'-diphosphoadenosine as coenzyme.
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以3,5-二磷酸腺苷为辅酶的硫酸盐在酚类化合物之间的转移。
DOI：
10.1016/s0021-9258(19)63710-5
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发表时间：
1957
期刊：
影响因子：
--
通讯作者：
F. Lipmann
中科院分区：
文献类型：
--
作者：
J. D. Gregory;F. Lipmann
硝基苯酯已广泛用于水解酶的测定，Huggins和Smith（1）将磷酸盐引入磷酸酶测定。硫酸盐最近被Roy（2）用于芳基硫酸酯酶的测定。然而，已经用各种酚作为硫酸盐受体研究了硫酸盐活化和转移（3-5）。当本实验室开始硫酸盐活化工作时，使用对硝基苯酚似乎是有利的，因为在酯化反应中有色阴离子消失，所以对硝基苯酚作为转移指示剂。在这些实验的过程中，进行了许多观察，这些观察表明活性硫酸盐（PAPS）1（6）和硝基苯酚之间的可逆反应。本文件详细论述了这种可逆性的各个方面。催化该反应的酶，苯酚磺基激酶，似乎具有相当广泛的活性，涵盖了大量的酚类化合物。然而，这是具体针对PAPS的。如果使用合理高浓度的对硝基苯基硫酸盐，则可以观察到逆反应，其中硫酸盐返回到残余PAP中。该系统可用于PAP的测定，并且如果提供额外的硫酸盐受体则特别有效，所述硫酸盐受体的硫酸盐电位远低于p-PAP的硫酸盐电位，并且所述酶然后催化硫酸盐从p-PAP转移到苯酚，这取决于PAP作为硫酸盐载体的存在。磺基激酶p-NP+苯酚_____f PAP p-NP+硫酸苯酯
Nitrophenyl esters have been used extensively for the determination of hydrolases, the phosphate having been introduced by Huggins and Smith (1) for phosphatase assay. The sulfate has been used lately in particular by Roy (2) for arylsulfatase determination. Sulfate activation and transfer, however, have been studied with various phenols as sulfate acceptors (3-5). When work on sulfate activation was started in this laboratory, it seemed of advantage to use p-nitrophenol, which behaves as a transfer indicator because of the disappearance of the colored anion on esterification. In the course of these experiments, a number of observations were made which indicated a reversible reaction between active sulfate (PAPS) l (6) and nitrophenol. The present paper deals in detail with various aspects of this reversibility. The enzyme, phenol sulfokinase, which catalyzes this reaction, appears to have a rather broad activity covering a large variety of phenolic compounds. It is, however, specific with regard to PAPS. If reasonably high concentrations of p-nitrophenyl sulfate are used, the reverse reaction can be observed in which sulfate is returned to the residue PAP. This system may be used for assay of PAP and is particularly effective if supplied with an additional sulfate acceptor, the sulfate potential of which is far below that of p-NPS, and the enzyme then catalyzes a transfer of sulfate from p-NPS to phenol, which is dependent on the presence of PAP as sulfate carrier. sulfokinase p-NPS+ phenol _____f PAP p-NP+ phenyl sulfate