Protective effects of sinomenine against LPS-induced inflammation in piglets
Protective effects of sinomenine against LPS-induced inflammation in piglets
复制标题
青藤碱对脂多糖诱导仔猪炎症的保护作用
DOI：
10.1016/j.micpath.2017.07.044
复制
发表时间：
2017-09-01
影响因子：
3.8
通讯作者：
Hu, Yiyi
中科院分区：
文献类型：
--
作者：
Yang, Haifeng;Jiang, Chunmao;Hu, Yiyi
本研究旨在探讨中草药青藤碱对仔猪的抗内毒素和抗炎作用。在高、中和低剂量青藤碱组中，仔猪最初用内毒素攻击（即，1 mg脂多糖（LPS）/kg）腹腔注射，3 h后肌肉注射青藤碱1、5或10 mg/kg。在药物对照组中，仔猪IP给予溶媒，3 h后IM给予10 mg/kg青藤碱，而LPS对照组中的仔猪用1 mg LPS/kg（IP）攻击，3 h后给予溶媒;幼稚对照仔猪IP给予生理盐水，然后仅IM。在LPS/媒介物注射后12、24和48小时，从小猪的前腔静脉收集血液样品。在试验期间记录临床体征，并通过ELISA试剂盒分析指数水平。结果显示青藤碱可以降低某些LPS诱导的毒性的发生率/严重程度，例如，细胞粘附、全身炎症和多器官功能障碍。总之，这些数据向我们表明，青藤碱可能有效地调节炎症反应，作为未来抗内毒素治疗的一部分。(C)2017爱思唯尔有限公司版权所有
The aim of this study was to investigate in piglets, the anti-endotoxin and anti-inflammatory effects of sinomenine, an agent commonly found in Chinese herbal medicines. In high-, middle- and low-dose sinomenine groups, piglets were initially challenged with endotoxin (i.e., 1 mg lipopolysaccharide (LPS)/kg) by intraperitoneal (IP) injection and, 3 h later, intramuscularly (IM) with sinomenine at 1, 5, or 10 mg/kg. In a drug control group, piglets were dosed IP with vehicle and 3 h late IM with 10 mg/kg sinomenine while those in an LPS control group were challenged with 1 mg LPS/kg (IP) and then vehicle 3 h later; naive control piglets were administered normal saline IP and then IM only. At 12, 24, and 48 h post-LPS/vehicle injection, blood samples were collected from the precaval vein of piglets. Clinical signs were recorded during the trial and index levels were analyzed by ELISA kits. The results revealed sinomenine could reduce the incidence/severity of certain LPS-induced toxicities, e.g., cell adhesion, systemic inflammation, and multiple organ dysfunction. Taken together, the data suggested to us that sinomenine might effectively be useful to regulate inflammatory responses as part of future anti-endotoxin therapies. (C) 2017 Elsevier Ltd. All rights reserved.