A comparison of morphologic features, flow cytometry, TCR-Vβ analysis, and TCR-PCR in qualitative and quantitative assessment of peripheral blood involvement by Sezary syndrome
A comparison of morphologic features, flow cytometry, TCR-Vβ analysis, and TCR-PCR in qualitative and quantitative assessment of peripheral blood involvement by Sezary syndrome
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DOI：
10.1309/25e9y7rray84htat
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Hanson, CA
中科院分区：
文献类型：
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作者：
Morice, WG;Katzmann, JA;Hanson, CA
各种实验室方法用于外周血(PB)Sezary细胞定量的优缺点尚未进行严格的比较。本研究对11例Sezary综合征(SS)患者外周血标本进行了手工Sezary细胞计数、定性和定量流式细胞术、T细胞受体(TCR)V-β流式细胞术和TCR聚合酶链式反应。反应性红皮病，6例真菌样肉芽肿，11例健康对照。这些方法在90%以上的SS病例中发现了肿瘤细胞。这些测试的诊断特异性各不相同：它们通过应用国际皮肤淋巴瘤协会提出的标准而得到加强。对6例SS患者的序列分析标本比较发现，尽管克隆细胞的绝对数量减少，但在某些情况下，这些细胞仍然构成了CD4+T细胞亚群的绝大多数，这表明定量亚群分析可能足以监测PB肿瘤负荷的变化。
The strengths and weaknesses of various laboratory methods for peripheral blood (PB) Sezary cell quantitation have not been compared rigorously. In this study, manual Sezary cell counting, qualitative and quantitative flow cytometry, T-cell receptor (TCR) V-beta flow cytometry, and TCR polymerase chain reaction were performed on PB specimens from 11 patients with Sezary syndrome (SS), 9 with. reactive erythroderma, 6 with mycosis fungoides, and 11 healthy control subjects. These methods identified neoplastic cells in more than 90% of SS cases. The diagnostic specificities of these tests varied: they were enhanced by applying criteria proposed by the International Society for Cutaneous Lymphoma. Comparison of sequentially analyzed specimens from 6 patients with SS revealed that although the absolute number of clonal cells was reduced, in some cases, these cells still constituted the vast majority of the CD4+ T-cell subset, suggesting that quantitative subset analysis might be sufficient to monitor changes in the PB tumor burden.