CHEMICAL DISSOLUTION AND IN VITRO RECONSTRUCTION OF SPONGE CELL ADHESIONS .1. ISOLATION AND FUNCTIONAL DEMONSTRATION OF COMPONENTS INVOLVED
CHEMICAL DISSOLUTION AND IN VITRO RECONSTRUCTION OF SPONGE CELL ADHESIONS .1. ISOLATION AND FUNCTIONAL DEMONSTRATION OF COMPONENTS INVOLVED
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DOI：
10.1016/0012-1606(63)90024-1
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发表时间：
1963-01-01
影响因子：
2.7
通讯作者：
HUMPHREYS, T
中科院分区：
文献类型：
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作者：
HUMPHREYS, T
本工作的目的是以分离的[单轴]海绵细胞聚集为实验系统[微小海绵、暗色海绵、海绵海绵、海绵海绵]为实验系统，研究海绵细胞的黏附机制。开发了一种利用无钙、无镁的冷海水对海绵进行化学解离的方法。这种分离产生的有活力的单个细胞能够聚集并发育成具有功能的海绵。旋转介导的聚集被用来研究海绵细胞聚集的各个方面。利用这些技术，为海绵细胞黏附建立了对二价阳离子钙和镁的特殊要求。低温被发现抑制了化学分离的细胞的粘附性，即使当二价阳离子被添加回细胞时也是如此。机械分离的细胞在低温下迅速聚集。这种差异被证明是由于海绵组织在化学解离过程中释放到上清液中的一种因子。当这一因子与二价阳离子一起被加回到化学分离的细胞中时，它们在低温下迅速黏附。这一因素是物种特有的，只导致来自同一物种的细胞的黏附。这些结果被解释为海绵细胞的黏附由三个基本成分组成：细胞表面、二价阳离子和细胞间物质。这些成分在化学解离过程中被分离，并能够自发地和物种特异性地重新组装，以改变表面上正常的细胞黏附。讨论了这些结果对细胞黏附模型的影响。
The purpose of this work was to study the mechanism of cell adhesion using aggregation of dissociated [monaxonic] sponge cells as the experimental system [Microciona prolifera, Haliclona occulata, Halichondria panicea, Cliona celata]. A method of chemical dissociation was developed for marine sponges using cold calcium- and magnesium-free sea water. The viable, single cells resulting from this dissociation were able to aggregate and develop into functional sponges. Rotation-mediated aggregation was used to study various aspects of the aggregation of sponge cells. Using these techniques, a specific requirement for the divalent cations calcium and magnesium was established for sponge cell adhesion. Low temperature was found to inhibit adhesion of chemically dissociated cells even when the divalent cations were added back to the cells. Mechanically dissociated cells aggregated rapidly at low temperatures. This difference was shown to be due to a factor released into the supernatant during chemical dissociation of sponge tissue. When this factor was added back to the chemically dissociated cells along with divalent cations, they adhered rapidly at low temperatures. This factor was species specific, causing adhesion only of cells from the same species. These results were interpreted to indicate that the sponge cell adhesion was composed of three basic components the cell surface, divalent cations, and an intercellular material. These components had been separated during chemical dissociation and were capable of spontaneously and species specifically reassembling themselves to reform an apparently normal cell adhesion. The implications of these results for models of cell adhesion are discussed.