Large dense-core vesicle exocytosis in pancreatic β-cells monitored by capacitance measurements
Large dense-core vesicle exocytosis in pancreatic β-cells monitored by capacitance measurements
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DOI：
10.1016/j.ymeth.2004.01.003
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发表时间：
2004-08-01
期刊：
影响因子：
4.8
通讯作者：
Rorsman, P
中科院分区：
文献类型：
--
作者：
Kanno, T;Ma, XS;Rorsman, P
本文讨论了当前使用的监测胞吐作用作为细胞电容变化的方法。详细介绍了溶液的组成、实验方案以及如何从生理学角度解释观察到的反应。我们通过自己在释放胰岛素的胰腺 P 细胞方面的工作中的例子来说明这些概念。最后，我们考虑将电容测量应用于完整胰岛中的内分泌细胞的可行性，其中细胞彼此电耦合。 (C) 2004 Elsevier Inc. 保留所有权利。
This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.