Adhesion of single bacterial cells in the micronewton range
Adhesion of single bacterial cells in the micronewton range
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DOI：
10.1073/pnas.0601705103
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发表时间：
2006-04-11
影响因子：
11.1
通讯作者：
Tang, JX
中科院分区：
文献类型：
--
作者：
Tsang, PH;Li, GL;Tang, JX
细菌对表面的黏附在环境、疾病和工业中起着至关重要的作用。在水生环境中，新月藻是水下表面的首批定殖者之一。利用显微操作技术，我们测量了单个C crescentus细胞通过它们的粘结剂固定器附着在硼硅酸盐基质上的粘附力。14个电池的剥离力在0.11~2.26 mN之间，平均0.59+/-0.62 mU N。通过有限元分析方法(将物体划分为小网格并计算所有单元的相关参数)计算应力分布，在接触中心区域，固定器与基材的粘结强度为>；68N/mm(2)。据我们所知，这种粘合强度是生物粘合剂迄今测量到的最强粘合强度。
The adhesion of bacteria to surfaces plays critical roles in the environment, disease, and industry. In aquatic environments, Caulobacter crescentus is one of the first colonizers of submerged surfaces. Using a micromanipulation technique, we measured the adhesion force of single C crescentus cells attached to borosilicate substrates through their adhesive holdfast. The detachment forces measured for 14 cells ranged over 0.11 to 2.26 mu N, averaging 0.59 +/- 0.62 mu N. Based on the calculation of stress distribution with the finite element analysis method (dividing an object into small grids and calculating relevant parameters for all of the elements), the adhesion strength between the holdfast and the substrate is > 68 N/mm(2) in the central region of contact. To our knowledge, this strength of adhesion is the strongest ever measured for biological adhesives.