Potato CONSTANS is involved in photoperiodic tuberization in a graft-transmissible manner
Potato CONSTANS is involved in photoperiodic tuberization in a graft-transmissible manner
复制标题
DOI：
10.1111/j.1365-313x.2012.04909.x
复制
发表时间：
2012-05-01
期刊：
影响因子：
7.2
通讯作者：
Suarez-Lopez, Paula
中科院分区：
文献类型：
--
作者：
Gonzalez-Schain, Nahuel D.;Diaz-Mendoza, Mercedes;Suarez-Lopez, Paula
CONSTANS（CO）参与植物发育过程的光周期控制，包括几个物种的开花和树木的季节性生长停止和出芽。有人提出，CO还可以影响马铃薯（马铃薯）（一种具有重要农业相关性的植物）块茎诱导的日长调节。为了解决这个问题，我们研究了CO在马铃薯中的作用。一个马铃薯CO样基因StCO被鉴定，并发现与以前报道的马铃薯功能未知的基因高度相似。马铃薯植株过表达StCO块茎晚于野生型植物弱诱导光周期。StCO沉默抑制和弱诱导光周期下促进块茎形成，但没有任何影响下强烈诱导短的日子，表明StCO抑制块茎形成的光周期依赖的方式。StCO对块茎诱导的影响通过嫁接传递。此外，StCO影响StBEL 5的mRNA水平，一种块茎形成启动子，其mRNA在马铃薯植物中长距离移动，以及StFT/StSP 6A，一种与FLOWERLOCUS T（FT）高度相似的蛋白质，其是其他物种中系统开花信号的关键组分。我们还发现StFT/StSP 6A转录水平与野生型植物中块茎形成的诱导相关。这些结果表明，StCO在光周期块茎化中起着重要作用，并且连同最近的证明，StFT/StSP 6A促进块茎化，表明CO/FT模块参与控制这个过程。此外，他们支持这样的概念，即StCO参与马铃薯中长距离调控信号的表达，就像CO在其他物种中一样。
CONSTANS (CO) is involved in the photoperiodic control of plant developmental processes, including flowering in several species and seasonal growth cessation and bud set in trees. It has been proposed that CO could also affect the day-length regulation of tuber induction in Solanum tuberosum (potato), a plant of great agricultural relevance. To address this question, we examined the role of CO in potato. A potato CO-like gene, StCO, was identified and found to be highly similar to a previously reported potato gene of unknown function. Potato plants overexpressing StCO tuberized later than wild-type plants under a weakly inductive photoperiod. StCO silencing promoted tuberization under both repressive and weakly inductive photoperiods, but did not have any effect under strongly inductive short days, demonstrating that StCO represses tuberization in a photoperiod-dependent manner. The effect of StCO on tuber induction was transmitted through grafts. In addition, StCO affected the mRNA levels of StBEL5 a tuberization promoter, the mRNA of which moves long distances in potato plants and StFT/StSP6A, a protein highly similar to FLOWERING LOCUS T (FT), which is a key component of systemic flowering signals in other species. We also found that StFT/StSP6A transcript levels correlate with the induction of tuber formation in wild-type plants. These results show that StCO plays an important role in photoperiodic tuberization and, together with the recent demonstration that StFT/StSP6A promotes tuberization, indicate that the CO/FT module participates in controlling this process. Moreover, they support the notion that StCO is involved in the expression of long-distance regulatory signals in potato, as CO does in other species.