GENETIC-POLYMORPHISM IN THE RABIES VIRUS NUCLEOPROTEIN GENE
GENETIC-POLYMORPHISM IN THE RABIES VIRUS NUCLEOPROTEIN GENE
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DOI：
10.1006/viro.1995.1285
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发表时间：
1995-06-01
期刊：
影响因子：
3.7
通讯作者：
BOURHY, H
中科院分区：
文献类型：
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作者：
KISSI, B;TORDO, N;BOURHY, H
为了比较狂犬病毒基因型的内在和外在遗传多样性，对来自世界各地的 69 个狂犬病病毒分离株进行了部分测序，并与 6 种狂犬病病毒基因型的 13 个代表性分离株进行了比较。对完整核蛋白（N）编码基因（1350个碱基）进行的系统发育关系分析表明，狂犬病病毒分离株均属于基因型1，并且根据其地理定位和起源物种，至少可以鉴定出11个系统发育谱系。这些谱系的分歧主要是由于同义突变的积累。稳定选择可能与宿主特异性有关，限制了氨基酸序列和抗原漂移。还对编码 N 蛋白氨基末端的高度可变的 400 个碱基区域或对应于 N mRNA 3' 端、基因间 N 磷蛋白 (M1) 区域和 M1 mRNA 5' 端的 93 个碱基非编码区进行了分析。这些较短的核苷酸序列被证明可以提供适合完成大型流行病学研究的系统发育数据，但稳健性较差。尽管后一种非编码序列的突变率比其相邻的编码 N 基因高 3.1 倍，但它遵循平行的进化模式。 (C) 1995 学术出版社
In an attempt to compare intrinsic and extrinsic genetic diversity of the lyssavirus genotypes, 69 rabies virus isolates from various part of the world were partially sequenced and compared to 13 representative isolates of the 6 lyssavirus genotypes. The analysis of their phylogenetic relationships, performed on the complete nucleoprotein (N) coding gene (1350 bases), established that the rabies virus isolates all belonged to genotype 1 and that at least 11 phylogenetic lineages could be identified in accordance with their geographical localization and species of origin. These lineages diverged mostly by the accumulation of synonymous mutations. Stabilizing selection, possibly related to host specificity, limits amino acid sequence and antigenic drift Analyses were also performed either on the highly variable 400-base region coding for the amino terminus of the N protein or on the 93-base noncoding region corresponding to the 3' end of the N mRNA, the intergenic N-phosphoprotein (M1) region, and the 5' end of the M1 mRNA. These shorter nucleotide sequences were shown to provide phylogenetic data suitable for the completion of large epidemiological studies, but with less robustness. This latter noncoding sequence, despite a 3.1 times higher mutation rate than its adjacent coding N gene, followed a parallel evolutionary pattern. (C) 1995 Academic Press, Inc.