In-utero transplantation of fetal liver haemopoietic stem cells in monkeys.
In-utero transplantation of fetal liver haemopoietic stem cells in monkeys.
复制标题
猴胎肝造血干细胞的宫内移植。
DOI：
10.1016/s0140-6736(89)92036-9
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Zanjani,ED
中科院分区：
文献类型：
--
作者：
Harrison,MR;Slotnick,RN;Crombleholme,TM;Golbus,MS;Tarantal,AF;Zanjani,ED
为了评价胎儿造血干细胞（HSC）宫内移植永久植入治疗先天性血红蛋白病的潜力，用来自胎儿肝脏的HSC移植胎猴。5只孕猴（孕60-62天）在孕59-68天子宫内腹腔注射来自异性供体的胎肝细胞（108- 109个细胞/kg估计胎受体体重）。通过外周血白细胞和骨髓的核型分析证实植入;在受体细胞中发现供体性别的细胞。供体细胞植入是明显的，在四个在子宫内造血干细胞移植受体在出生时。植入涉及淋巴（2.9 - 8.0%供体细胞）、红系（5.3 - 12.5%）和髓系（8.5 - 15.4%）谱系，并持续长达2年，无移植物抗宿主病证据。
To evaluate the potential of in-utero transplantation of fetal haemopoietic stem cells (HSCs) for permanent engraftment as a treatment of congenital haemoglobinopathies, fetal rhesus monkeys were transplanted with HSCs derived from fetal livers. Five pregnant monkeys (60-62 days' gestation) were given an in-utero intraperitoneal injection of fetal liver cells (108-109cells/kg estimated fetal recipient body weight) derived from opposite sex donors at 59-68 days' gestation. Engraftment was confirmed by karyotype analysis of peripheral blood leucocytes and bone marrow; cells of donor sex were found among the recipient cells. Donor cell engraftment was apparent in four of five in-utero HSC transplant recipients at birth. Engraftment involved lymphoid (2·9-8·0% donor cells), erythroid (5·3-12·5%), and myeloid (8·5-15·4%) lineages and has persisted for up to 2 years without evidence of graft-versus-host disease.