Antigenicity of the infected-erythrocyte and merozoite surfaces in Falciparum malaria.
Antigenicity of the infected-erythrocyte and merozoite surfaces in Falciparum malaria.
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DOI：
10.1084/jem.150.5.1241
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发表时间：
1979-11-01
影响因子：
15.3
通讯作者：
Reese, R T
中科院分区：
文献类型：
--
作者：
Langreth, S G;Reese, R T
本文检测了恶性疟原虫感染的猕猴和人红细胞膜上恶性疟原虫引起的结构改变的抗原性。抗血清是从对疟疾免疫的猴子身上获得的。结合的抗体被证明定位在人和猴子来源的感染红细胞的旋钮突起上，以及来自体外和体内感染的红细胞上。因此，恶性疟原虫感染在人和猴子的红细胞表面产生了相似的抗原性变化。未感染的红细胞和所有无结节感染的红细胞均不结合免疫血清的抗体。来自不同地理区域的恶性疟原虫菌株在体外培养于具有共同抗原性的人红细胞诱导结构(旋钮)中。当与免疫血清孵育时，裂殖子通过其细胞层的交联性而凝集。铁蛋白标记的抗体在同源和异种寄生虫的衣壳上结合力很强，表明这些虫株的裂殖子表面具有共同的抗原。
The antigenicity of altered structures induced by Plasmodium falciparum in the membranes of infected Aotus monkey and human erythrocytes was examined. Antisera were obtained from monkeys made immune to malaria. Bound antibodies were shown to be localized on the knob protrusions of infected erythrocytes of both human and monkey origin and from both in vitro and in vivo infections. Therefore, P. falciparum infection has produced similar antigenic changes in the erythrocyte surfaces of both man and monkey. Uninfected erythrocytes and all knobless-infected erythrocytes bound no antibody from immune sera. Strains of P. falciparum from widely different geographic areas that were cultured in vitro in human erythrocytes induced structures (knobs) which have common antigenicity. Merozoites were agglutinated by cross-linking of their cell coats when incubated with immune sera. The binding of ferritin-labeled antibody was heavy on the coats of both homologous and heterologous strains of the parasite, indicating that the merozoite surfaces of these strains share common antigens.