Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: Possible role in reproduction
Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: Possible role in reproduction
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DOI：
10.1016/j.fertnstert.2005.05.019
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发表时间：
2005-11-01
影响因子：
6.7
通讯作者：
D'Aniello, A
中科院分区：
文献类型：
--
作者：
D'Aniello, G;Ronsini, S;D'Aniello, A
目的：测定可育和不育供者精浆和精子中d -天冬氨酸（D-Asp）含量。设计：前瞻性观察研究。单位：意大利萨勒诺“S. Luca”医院人类生殖病理生理科和意大利那不勒斯“A Dohrn”动物站神经生物学和比较生理学科。患者：10例正常精子，10例少精，10例无精子（非梗阻性）。干预措施：d -天冬氨酸通过纯化精浆和分离精子上的特定酶高效液相色谱法和使用光学和电子显微镜技术的免疫组织化学方法测定。主要观察指标：精浆和离体精子中D-Asp的浓度；D-Asp在顶体和细胞核中的亚细胞定位。结果：低弱异精子供者精浆和精子中D-Asp的浓度显著降低。在正常精子供者的精液中，D-Asp的浓度为80 +/- 12 nmol/mL (10.4 +/- 1.5 μ g/ mL)，而在低弱异精子供者的精液中，D-Asp的浓度为26 +/- 6 nmol/mL，在无精子供者的精液中，D-Asp的浓度为12 +/- 1.5 nmol/mL。在正常精子供者的精子中，D-Asp的浓度为每个精子130 +/- 15 fmol （17.0 +/- 1.96 ng /个精子），而在低弱异精子供者的精子中，D-Asp的浓度为每个精子60.5 +/- 5.0 fmol。与D-Asp相比，分析的其他d -氨基酸在精浆或精子中的浓度不显著。结论：d -天冬氨酸存在于人类精浆和精子中，与男性生育能力有关。
Objective: To determine D-aspartic acid (D-Asp) in human seminal plasma and spermatozoa in fertile and infertile donors.Designs: Prospective observation study.Setting: Department of Pathophysiology for Human Reproduction, Hospital "S. Luca" Salerno Italy, and Department of Neurobiology and Comparative Physiology, Zoological Station "A Dohrn," Naples, Italy.Patient(s): Ten normospermic, 10 oligoasthenoteratospermic, and 10 azoospermic (nonobstructive) men.Intervention(s): D-aspartic acid was determined by a specific enzymatic high-performance liquid chromatography method on purified seminal plasma and on isolated spermatozoa and by an immunohistochemical method using light and electronic microscopic techniques.Main Outcome Measure(s): Concentration of D-Asp in seminal plasma and in isolated spermatozoa; subcellular localization of D-Asp in the acrosome and nucleus.Result(s): The concentration of D-Asp in seminal plasma and in spermatozoa was significantly reduced in oligoasthenoteratospermic donors. In the seminal fluid of normospermic donors, D-Asp occurs at a concentration of 80 +/- 12 nmol/mL semen (10.4 +/- 1.5 mu g/ml), whereas 26 +/- 6 nmol/mL semen were found in oligoasthenoteratospermic donors, and 12 +/- 1.5 nmol/mL semen were found in azoospermic donors. In spermatozoa from normospermic donors, D-Asp occured at a concentration of 130 +/- 15 fmol per spermatozoa (17.0 +/- 1.96 ng per spermatozoa), vs. 60.5 +/- 5.0 fmol per spermatozoa from oligoasthenoteratospermic subjects. Other D-amino acids analyzed were not present in seminal plasma or in spermatozoa in a significant concentration compared with D-Asp.Conclusion(s): D-aspartic acid occurs in human seminal plasma and spermatozoa and is implicated in male fertility.