Role of Vfr in the regulation of antifungal compound production by Pseudomonas fluorescens FD6
Role of Vfr in the regulation of antifungal compound production by Pseudomonas fluorescens FD6
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DOI：
10.1016/j.micres.2016.04.013
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发表时间：
2016-01-01
影响因子：
6.7
通讯作者：
Liu, Fengquan
中科院分区：
文献类型：
--
作者：
Zhang, Qingxia;Ji, Yanyan;Liu, Fengquan
荧光假单胞菌FD6已被证明具有许多有益的性状，参与对真菌植物病原体的生物防治，如灰葡萄孢菌和果糖念珠菌。毒力因子调节剂（vir毒力因子调节剂）是革兰氏阴性细菌（如人类病原体铜绿假单胞菌）高度保守的全局调节剂，是许多重要毒力性状表达所必需的。Vfr在调节生物防治性状中的作用，如拮抗细菌产生抗生素来控制真菌病原体，尚未阐明。本研究研究了来自荧光假单胞菌FD6的vfr突变体的作用，以更好地了解与该细菌相关的一些重要生物防治性状的调控。生化研究表明，在vfr突变体中，抗生素2,4-二乙酰基间苯三酚、吡咯硝丁和吡咯啉的产量显著增加。vfr突变还增加了生物膜的产生、游泳运动和外多糖相关基因（pelA、pslA和pslB）转录本的表达，但减少了蛋白酶的产生。接种后7和21 d， vfr突变体在小麦根际和根尖的定植量均高于野生型。这些发现表明，Vfr调节荧光假单胞菌FD6生物防治潜能中涉及的几个关键性状的表达和重要抗生素的产生。(C) 2016 Elsevier GmbH版权所有。
Pseudomonas fluorescens FD6 has been shown to possess many beneficial traits involved in the biocontrol of fungal plant pathogens, such as Botrytis cinerea and Monilinia fructicola. Vfr (virulence factor regulator) a highly conserved global regulator of gram-negative bacteria, such as the human pathogen Pseudomonas aeruginosa, is required for the expression of many important virulence traits. The role of Vfr in the regulation of biocontrol traits, such as the production of antibiotics to control fungal pathogens by antagonistic bacteria, has not been elucidated. This study investigated the effect of a vfr mutant derived from P. fluorescens FD6 to better understand the regulation of some important biocontrol traits associated with the bacterium. Biochemical studies indicated that the production of the antibiotics 2,4-diacetylphloroglucinol, pyrrolnitrin and pyoluteorin, was markedly enhanced in the vfr mutant. The vfr mutation also increased biofilm production, swimming motility and the expression of exopolysaccharide-associated gene (pelA, pslA and pslB) transcripts, but reduced protease production. Wheat rhizosphere and root tip colonization by the vfr mutant was higher than that by the wild type at 7 and 21 days after inoculation. These findings demonstrate that Vfr modulates the expression of several key traits and the production of important antibiotics involved in the biocontrol potential of P. fluorescens FD6. (C) 2016 Elsevier GmbH. All rights reserved.