A cloning vector allowing excision of inserts with original termini irrespective of their sequence.
A cloning vector allowing excision of inserts with original termini irrespective of their sequence.
复制标题
一种克隆载体，允许切除具有原始末端的插入片段，无论其序列如何。
DOI：
10.1093/nar/16.16.8182
复制
发表时间：
1988
影响因子：
14.9
通讯作者：
C. Bostock
中科院分区：
文献类型：
--
作者：
Z. Pan;C. Bostock
在许多克隆过程中，能够回收克隆的插入片段及其精确的原始末端而不依赖于形成限制酶回文识别序列的一部分的末端序列是有用的。例如，DNA通常被克隆到载体多接头的SmaI位点，但由于这通常会破坏SmaI位点，因此插入片段的切除必须通过原始多接头中SmaI位点侧翼的位点进行。从这样的过程中切除的插入物将在两个末端携带额外的核苷酸。为了避免这个问题，我们通过在SmaI位点插入寡核苷酸来修饰pUC 19（1）以产生pZP19。
In many cloning procedures it would be useful to be able to recover cloned inserts with their exact original termini without beintg dependent on terminal sequences forming part of a palindromic recognition sequence of a restriction enzyme. For eaxample,.,DNAs-are often cloned into the SmaI site of a vector's polylinker, but since this usually destroys the SmaI site, excision of the insert would have to be via sites which flank the SmaI site in the original polylinker. Excised inserts from such a procedure would carry additional nucleotides at both termini. To avoid this problem we have modified pUC19(l) by inserting an oligonucleotide into the SmaI site to create pZP19.