Network pharmacology to dissect the mechanisms of Yinlai Decoction for pneumonia
Network pharmacology to dissect the mechanisms of Yinlai Decoction for pneumonia
复制标题
网络药理学剖析银来汤治疗肺炎作用机制
DOI：
10.1186/s12906-020-02954-z
复制
发表时间：
2020-06-03
影响因子：
3.9
通讯作者：
Gu, Xiaohong
中科院分区：
文献类型：
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作者：
Xu, Jingnan;Bai, Chen;Gu, Xiaohong
研究背景肺炎是一种常见的呼吸系统疾病，给医疗系统带来了巨大的经济负担。然而，由于耐药性和副作用，目前肺炎的治疗选择有限。本课题组前期研究初步证实了临床上治疗肺炎的常用方剂银来汤（Yinglaingtang，YD）对炎症因子表达的调节作用，但其机制尚不清楚。在TCMSP数据库中获得了YD的活性成分，并通过目标垂钓法收集了这些活性成分的潜在靶标。利用TTD、Drugbank、HPO、OMIM、CTD建立肺炎相关靶点数据库。基于YD和肺炎之间的匹配靶点，通过STRING构建PPI网络，分析这些靶点之间的相互作用，然后输入Cytoscape进行进一步的拓扑分析。大卫和KEGG用于GO和途径富集分析。结果共鉴定出68个有效成分、103个潜在作用靶点和8条可能发挥作用的相关通路。使用Cytoscape构建了三个网络，即中药成分网络、YD肺炎靶标网络和中药成分-YD肺炎靶标网络。结论网络分析表明，YD通过调节宿主免疫炎症反应、血管生成和血管通透性、气道上皮细胞屏障功能、激素释放和细胞生长、增殖、凋亡等，减轻肺炎症状和体征。
BackgroundPneumonia is a common respiratory disorder, which brings an enormous financial burden to the medical system. However, the current treatment options for pneumonia are limited because of drug resistance and side effects. Our previous study preliminarily confirmed that Yinlai Decoction (YD), a common prescription for pneumonia in clinical practice, can regulate the expression of inflammatory factors, but the mechanisms are unknown yet.MethodsIn our work, a method named network pharmacology was applied, which investigated the underlying mechanisms of herbs based on a variety of databases. We obtained bioactive ingredients of YD on TCMSP database and collected potential targets of these ingredients by target fishing. Then the pneumonia-related targets database was built by TTD, Drugbank, HPO, OMIM, and CTD. Based on the matching targets between YD and pneumonia, the PPI network was built by STRING to analyze the interactions among these targets and then input into Cytoscape for further topological analysis. DAVID and KEGG were utilized for GO and pathway enrichment analysis. Then rat model based on LPS stimulated pneumonia was used to verify the possible mechanism of YD in treating pneumonia.ResultsSixty-eight active ingredients, 103 potential targets and 8 related pathways, which likely exert a number of effects, were identified. Three networks were constructed using Cytoscape, which were herb-component-network, YD-pneumonia target network, and herb-component-YD target-pneumonia network. YD was verified to treat LPS-induced pneumonia by regulating the inflammatory factor IL-6, which was a predicted target.ConclusionNetwork analysis indicated that YD could alleviate the symptoms and signs of pneumonia through regulating host immune inflammatory response, angiogenesis and vascular permeability, the barrier function of the airway epithelial cells, hormone releasing and cell growth, proliferation, and apoptosis.