Translation calibration of inverse-kappa goniometers in macromolecular crystallography.
Translation calibration of inverse-kappa goniometers in macromolecular crystallography.
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DOI：
10.1107/s0108767311004831
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Ravelli RB
中科院分区：
文献类型：
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作者：
Brockhauser S;White KI;McCarthy AA;Ravelli RB
一种快速、易于执行的平移校准程序已被开发出来，用于EMBL/ESRF mini-κ测角器头和其他专为大分子晶体学设计的反κ测角器。定期校准确保了依赖于晶体重定向中的许多自由度的实验的精度。精确而方便的晶体再取向在大分子晶体学中具有重要的实验意义。ESRF/EMBL mini-κ等多轴测角仪的开发需要相应开发可用于此类设备的设置、维护和故障排除的校准程序。虽然传统的多轴测角仪要求所有旋转轴与样品位置的唯一点相交，但最近开发的用于MX中样品重定向的小型化仪器并不受此限制。然而，样品必须在方向改变后重新居中。为了克服这种不便并允许使用多轴测角仪，而没有使所有轴相交于同一点的基本限制，已经为这种仪器开发了自动平移校正协议。它需要关于旋转轴的方向和位置的精确信息。为了测量和提供这些信息，还开发了一种通用的、易于执行的平移校准（TC）程序。在ESRF的大多数MX光束线上进行了TC程序，并给出了一些结果供参考。
A rapid, easy-to-perform translation calibration procedure has been developed for use with the EMBL/ESRF mini-κ goniometer head and for other inverse-kappa goniometers designed for macromolecular crystallography. Regular calibration ensures the precision of experiments that rely on many degrees of freedom in crystal reorientation. Precise and convenient crystal reorientation is of experimental importance in macromolecular crystallography (MX). The development of multi-axis goniometers, such as the ESRF/EMBL mini-κ, necessitates the corresponding development of calibration procedures that can be used for the setup, maintenance and troubleshooting of such devices. While traditional multi-axis goniometers require all rotation axes to intersect the unique point of the sample position, recently developed miniaturized instruments for sample reorientation in MX are not as restricted. However, the samples must always be re-centred following a change in orientation. To overcome this inconvenience and allow the use of multi-axis goniometers without the fundamental restriction of having all axes intersecting in the same point, an automatic translation correction protocol has been developed for such instruments. It requires precise information about the direction and location of the rotation axes. To measure and supply this information, a general, easy-to-perform translation calibration (TC) procedure has also been developed. The TC procedure is routinely performed on most MX beamlines at the ESRF and some results are presented for reference.