Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis
Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis
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DOI：
10.1128/iai.72.10.6032-6039.2004
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Shankar, N
中科院分区：
文献类型：
--
作者：
Tendolkar, PM;Baghdayan, AS;Shankar, N
肠球菌在人类生态学中扮演着双重角色。它们作为胃肠道的寄生生物，也是多重耐药性医院获得性感染的主要原因。许多医院感染是由微生物形成生物膜的能力引起的。肠球菌生物膜形成的分子机制现在才开始被理解。肠球菌表面蛋白，Esp，已被报道有助于粪肠球菌生物膜的形成。最近的研究表明，肠球菌形成的生物膜独立于Esp的表达。为了更准确地确定Esp在E.在粪肠球菌生物膜形成中，Esp在遗传上明确定义的天然Esp-deficient菌株的细胞表面上表达，并且比较了同基因Esp-positive和Esp-deficient菌株的生物膜形成能力。结果显示，Esp表达导致生物膜形成的显著增加，与测试的菌株无关。发现Esp对生物膜形成的贡献在0.5%（wt/vol）或更高葡萄糖的存在下最显著。这些结果明确地将Esp定义为E.形成生物膜。
Enterococci play a dual role in human ecology. They serve as commensal organisms of the gastrointestinal tract and are also leading causes of multiple antibiotic-resistant hospital-acquired infection. Many nosocomial infections result from the ability of microorganisms to form biofilms. The molecular mechanisms involved in enterococcal biofilm formation are only now beginning to be understood. Enterococcal surface protein, Esp, has been reported to contribute to biofilm formation by Enterococcus faecalis. Recent studies have shown that enterococci form biofilms independently of Esp expression. To precisely determine what role Esp plays in E. faecalis biofilm formation, Esp was expressed on the cell surface of genetically well-defined, natively Esp-deficient strains, and isogenic Esp-positive and Esp-deficient strains were compared for their biofilm-forming ability. The results show that Esp expression leads to a significant increase in biofilm formation, irrespective of the strain tested. The contribution of Esp to biofilm formation was found to be most pronounced in the presence of 0.5% (wt/vol) or greater glucose. These results unambiguously define Esp as a key contributor to the ability of E. faecalis to form biofilms.