Prodrug activation gated by a molecular "OR" logic trigger
Prodrug activation gated by a molecular "OR" logic trigger
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DOI：
10.1002/anie.200500842
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Shabat, D
中科院分区：
文献类型：
--
作者：
Amir, RJ;Popkov, M;Shabat, D
通用的方法。薄层色谱（TLC）：默克60 F254硅胶板：用紫外线照射和/或用25 g磷酸钼酸，10 g Ce (SO4) 2·H2O， 60 mL conc溶液处理，可见化合物。H2SO4和940 mL H2O，然后用12g 2,4 -二硝基苯肼溶液在60 mL conc中加热和/或染色。H2SO4, 80 mL H2O和200 mL 95% EtOH，加热。-闪蒸色谱法（FC）：默克60硅胶（粒径0.040-0.063 mm），括号内给出洗脱液。1H核磁共振谱测量使用布鲁克Avance工作在200 MHz。化学位移以相对于TMS的δ表示（δ= 0 ppm），耦合常数J以Hz表示。除另有说明外，在室温下以CDCl3为溶剂记录光谱。所有常规试剂，包括盐和溶剂，均购自Aldrich （Milwaukee， MN）。
General methods. Thin layer chromatography (TLC): silica gel plates Merck 60 F254: compounds were visualized by irradiation with UV light and/or by treatment with a solution of 25 g phosphomolybdic acid, 10 g Ce (SO4) 2· H2O, 60 mL conc. H2SO4 and 940 mL H2O followed by heating and/or by staining with a solution of 12 g 2, 4-dinitrophenylhydrazine in 60 mL conc. H2SO4, 80 mL H2O and 200 mL 95% EtOH followed by heating.–Flash chromatography (FC): silica gel Merck 60 (particle size 0.040-0.063 mm), eluent given in parentheses. 1H NMR spectra were measured using Bruker Avance operated at 200 MHz. The chemical shifts are expressed in δ relative to TMS (δ= 0 ppm) and the coupling constants J in Hz. The spectra were recorded in CDCl3 as solvent at room temperature unless stated otherwise. All general reagents, including salts and solvents, were purchased from Aldrich (Milwaukee, MN).