Current insights into the microbial degradation for pyrethroids: strain safety, biochemical pathway, and genetic engineering.
Current insights into the microbial degradation for pyrethroids: strain safety, biochemical pathway, and genetic engineering.
复制标题
目前对拟除虫菊酯微生物降解的见解：菌株安全性、生化途径和基因工程。
DOI：
10.1016/j.chemosphere.2021.130542
复制
发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Shuliang Liu
中科院分区：
文献类型：
--
作者：
Tianye Zhao;Kaidi Hu;Jianlong Li;Yuanting Zhu;Aiping Liu;K. Yao;Shuliang Liu
拟除虫菊酯作为一种生物杀虫剂，对非靶标生物有明显的毒副作用。PYR及其一般毒性中间体3-苯氧基苯甲酸（3-PBA）最近在人类中显示出较高的检出率/水平，饮食被确定为主要暴露途径。微生物矿化已经成为解决这种不断升级的问题的一种通用策略。本文从菌种安全性、应用及表面活性剂等方面对PYR和3-PBA的生物降解进行了综述。已经报道了许多PYR降解微生物，其中少数专注于3-PBA。大多数菌株来自污染场地，而几种微生物食品培养物（MFC）已被调查。MFC如芽孢杆菌属（Bacillusspp.）和在PYRs降解微生物库中占主导地位的Pyrs是农产品采后过程中解毒的适用候选者。随后，我们讨论了承诺的降解步骤，其中负责PYRs酯键断裂的水解酶和负责3-PBA二苯醚键断裂的加氧酶起着至关重要的作用。最后，概述了关键酶基因的全面信息沿着与有关基因克隆的方法。细胞色素P450单加氧酶（Cytochrome P450 monooxygenases，CYP 450）是一种具有二苯醚断裂能力的酶。新发展的组学已成为一种可行的基因和酶挖掘技术。为了实现饲料和食品中PYRs的矿化，筛选富含相关酶的MFC并构建MFC衍生的转基因微生物（GSTs）具有很大的潜力。
As a biologically inspired insecticide, pyrethroids (PYRs) exert evident toxic side effects on non-target organisms. PYRs and their general toxic intermediate 3-phenoxybenzoic acid (3-PBA) have shown high detection rates/levels in human beings recently, for which diet was identified as the major exposure route. Microbial mineralization has emerged as a versatile strategy in addressing such escalating concern. Herein, PYRs and 3-PBA biodegradation with regards to strain safety, application and surfactant were summarized. Numerous PYRs-degrading microbes have been reported yet with a minority focused on 3-PBA. Most isolates were from contaminated sites while several microbial food cultures (MFCs) have been investigated. MFCs such asBacillusspp. andAspergillusspp. that dominate in PYRs-degrading microbial pools are applicable candidates for agricultural by-products detoxification during the postharvest process. Subsequently, we discussed committed degradation steps, wherein hydrolase responsible for PYRs ester linkage cleavage and oxygenase for 3-PBA diphenyl ether bond rupture play vital roles. Finally, comprehensive information of the key enzyme genes is outlined along with methodologies concerning gene cloning. Cytochrome P450 monooxygenases (CYP) is competent for diphenyl ether scission. Newly-developed omics has become a feasible gene and enzyme mining technology. To achieve PYRs mineralization in feed and food commodities, the screening of MFCs rich in related enzymes and the construction of MFCs-derived genetically modified microbes (GMMs) exhibit great potential considering the safety issues.