Cellular distribution of myosin-V in the guinea pig cochlea
Cellular distribution of myosin-V in the guinea pig cochlea
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DOI：
10.1023/a:1018523827852
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发表时间：
1997-02-01
期刊：
影响因子：
--
通讯作者：
Kachar, B
中科院分区：
文献类型：
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作者：
Coling, DE;Espreafico, EM;Kachar, B
最近，肌球蛋白-VI 和肌球蛋白-VII 被鉴定为小鼠突变和导致严重听力损失的人类综合征中的缺陷基因，揭示了非常规肌球蛋白对听力的重要性。另一类新型肌球蛋白 (V) 与神经元和其他分泌细胞中细胞内囊泡的运输有关。我们使用亲和纯化的抗体来确定肌球蛋白-V 在豚鼠内耳中的定位。在耳蜗的感觉上皮中，肌球蛋白-V 表位在神经元和支持细胞中被识别。神经元标记在内毛细胞和外毛细胞的传入神经支配中最为强烈。标记的支持细胞是 Hensen 和 Deiters 细胞，以及内缘、内指骨、内沟和齿间细胞。在耳蜗的血管组织中，我们观察到血管纹中间细胞以及纹和螺旋突起之间的边界细胞的染色。在 I 型前庭毛细胞上观察到传入圣杯神经末梢的染色。结果表明，与肌球蛋白 VI 和 VIII 一样，肌球蛋白-V 定位于对听觉功能可能至关重要的位置。
The importance of unconventional myosins to hearing has recently been revealed by the identification of myosins-VI and -VII as the defective genes in mouse mutations and in a human syndrome which lead to profound hearing loss. Another class of novel myosins (V) has been implicated in the trafficking of intracellular vesicles in neurons and other secretory cells. We used affinity-purified antibodies to determine the localization of myosin-V in the guinea pig inner ear. In the sensory epithelium of the cochlea, myosin-V epitopes were recognized in neuronal and supporting cells. Neuronal labelling was most intense in the afferent innervation of inner and outer hair cells. Supporting cells labelled were cells of Hensen and Deiters, and inner border, inner phalangeal, inner sulcus and interdental cells. In the vascular tissue of the cochlea, we observed staining of intermediate cells of the stria vascularis and of border cells between the stria and the spiral prominence. Staining of afferent chalice nerve endings was observed on type I vestibular hair cells. The results suggest that, like myosins VI and VIII, myosin-V is localized in positions that may be critical to auditory function.