Formation of water-in-CO(2) microemulsions with non-fluorous surfactant Ls-54 and solubilization of biomacromolecules.
Formation of water-in-CO(2) microemulsions with non-fluorous surfactant Ls-54 and solubilization of biomacromolecules.
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DOI：
10.1002/1521-3765(20020315)8:6
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发表时间：
2002-03
期刊：
影响因子：
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通讯作者：
Juncheng Liu;B. Han;Jianling Zhang;Gan‐zuo Li;Xiaogang Zhang;Jun Wang;B. Dong
中科院分区：
文献类型：
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作者：
Juncheng Liu;B. Han;Jianling Zhang;Gan‐zuo Li;Xiaogang Zhang;Jun Wang;B. Dong
测定了Ls-54表面活性剂在超临界CO(2)中的溶解度。结果表明，该表面活性剂是一种非氟、非硅氧烷的非离子表面活性剂，但在SC CO(2)中具有较高的可溶性，可形成CO(2)水微乳。微乳的高溶解度和形成的主要原因可能是表面活性剂具有4个亲CO(2)基团（环氧丙烷）和5个亲水基团（环氧乙烷），其分子量相对较低。本研究结果为CO(2)可溶性非氟和非硅氧烷表面活性剂的设计提供了有用的信息。CO(2)/Ls-54/H(2)O体系的相行为、溶剂致色探针研究和溶菌酶的紫外光谱证明了SC CO(2)微乳中存在水畴。采用同步辐射小角x射线散射的方法获得了Ls-54基co - 2水反胶束的结构信息。在限定的小q范围内（0.022-0.040 a(-1)），通过Guinier图(ln I(q) vs q(2))，得到了不同压力和水与表面活性剂的摩尔比W(0)在20.4-25.2 a范围内的反胶束半径。
The solubility of Ls-54 surfactant in supercritical CO(2) was determined. It was found that the surfactant was highly soluble in SC CO(2) and the water-in-CO(2) microemulsions could be formed, despite it being a non-fluorous and non-siloxane nonionic surfactant. The main reasons for the high solubility and formation of the microemulsions may be that the surfactant has four CO(2)-philic groups (propylene oxide) and five hydrophilic groups (ethylene oxide) and its molecular weight are relatively low. The results of this work provide useful information for designing CO(2)-soluble non-fluorous and non-siloxane surfactants. The phase behavior of the CO(2)/Ls-54/H(2)O system, solvatochromic probe study, and the UV spectrum of lysozyme proved the existence of water domains in the SC CO(2) microemulsions. The method of synchrotron radiation small-angle X-ray scattering was used to obtain the structural information on the Ls-54 based water-in-CO(2) reverse micelles. By using the Guinier plot (ln I(q) versus q (2)) on the data sets in a defined small q range (0.022-0.040 A(-1)), the radii of the reverse micelles were obtained at different pressures and molar ratio of water to surfactant, W(0), which were in the range of 20.4-25.2 A.