Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, andNosema ceranaeInfection
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, andNosema ceranaeInfection
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DOI：
10.1007/s00248-020-01538-1
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发表时间：
2020-07-15
影响因子：
3.6
通讯作者：
Antunez, K.
中科院分区：
文献类型：
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作者：
Castelli, L.;Branchiccela, B.;Antunez, K.
蜜蜂是重要的传粉者，在自然生态和农业环境中发挥着重要作用。蜜蜂群体的损失事件在世界范围内报道，并已与不同的害虫和病原体，农药暴露，营养压力。这种营养压力与单一种植面积的增加有关，这导致花粉可用性和多样性的减少。在这项研究中，我们研究了在实验室条件下，营养应激是否会影响蜜蜂肠道微生物群，蜜蜂免疫力和微孢子虫感染。与食用多花花粉相反，食用巨桉花粉被用作营养不良的饮食来研究营养应激。用大桉饲喂的蜜蜂中，意大利乳杆菌和蜜蜂乳杆菌（分别为Firm-4和Firm-5）和双歧杆菌的丰度较低。蜜蜂巴尔通体的丰度也比用多花花粉喂养的蜜蜂高。除了营养胁迫对蜜蜂微生物群的影响外，它还降低了卵黄蛋白原和免疫相关基因（葡萄糖氧化酶，透明质酸和溶菌酶）的表达水平。巨桉有利于微孢子虫的增殖。这些结果表明，营养应激影响蜜蜂肠道微生物群，对蜜蜂免疫力和病原体发育产生影响。这些结果可能有助于了解现代农业对蜜蜂健康的影响。
Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection byNosema ceranae, under laboratory conditions. Consumption ofEucalyptus grandispollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed withEucalyptus grandispollen showed a lower abundance ofLactobacillus melliferandLactobacillus apis(Firm-4 and Firm-5, respectively) andBifidobacteriumspp. and a higher abundance ofBartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally,Eucalyptus grandispollen favored the multiplication ofNosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.