Acute neuroactive drug exposures alter locomotor activity in larval zebrafish
Acute neuroactive drug exposures alter locomotor activity in larval zebrafish
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DOI：
10.1016/j.ntt.2009.04.066
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Padilla, S.
中科院分区：
文献类型：
--
作者：
Irons, T. D.;MacPhail, R. C.;Padilla, S.
作为开发有毒化学品优先排序的快速体内筛选的一部分，我们已经开始通过评估作用于中枢神经系统的原型药物的急性作用来表征斑马鱼（Danio rerio）幼虫的运动活动。 剂量并减少高剂量时的运动。将野生型幼虫单独维持在26℃的96孔微量滴定板中，在14:10小时的光:暗循环下，在0830小时开灯。受精后6天，通过浸泡向幼虫施用乙醇(1-41％v/v)、硫酸d-苯丙胺(0.1-20.0μM)或盐酸可卡因(0.2-50.0μM)。从暴露 20 分钟开始，使用 10 分钟交替的光（可见光）和暗（红外光）周期评估每只动物的运动能力，持续 70 分钟。低浓度的乙醇和 d-苯丙胺会增加活性，而较高浓度的所有三种药物都会降低活性。由于乙醇效应主要发生在光照期间，而 d-苯丙胺和可卡因效应发生在黑暗时期，因此交替照明条件被证明是有利的。这些结果表明斑马鱼幼虫对神经活性药物敏感，其运动反应与哺乳动物相似。由爱思唯尔公司出版
As part of the development of a rapid in vivo screen for prioritization of toxic chemicals, we have begun to characterize the locomotor activity of zebrafish (Danio rerio) larvae by assessing the acute effects of prototypic drugs that act on the central nervous systen-L Initially, we chose ethanol, d-amphetamine, and cocaine, which are known, in mammals, to increase locomotion at low doses and decrease locomotion at higher doses. Wild-type larvae were individually maintained in 96-well microtiter plates at 26 degrees C, under a 14:10 h light:dark cycle, with lights on at 0830 h. At 6 days post-fertilization, ethanol (1-41% v/v), d-amphetamine sulfate (0.1-20.0 mu M) or cocaine hydrochloride (0.2-50.0 mu M) were administered to the larvae by immersion. Beginning 20 min into the exposure, locomotion was assessed for each animal for 70 min using 10-minute, alternating light (visible light) and dark (infrared light) periods. Low concentrations of ethanol and d-amphetamine increased activity, while higher concentrations of all three drugs decreased activity. Because ethanol effects occurred predominately during the light periods, whereas the d-amphetamine and cocaine effects occurred during the dark periods, alternating lighting conditions proved to be advantageous. These results indicate that zebrafish larvae are sensitive to neuroactive drugs, and their locomotor response is similar to that of mammals. Published by Elsevier Inc.