Nuclear factor-κB activity in T cells from patients with rheumatic diseases:: A preliminary report
Nuclear factor-κB activity in T cells from patients with rheumatic diseases:: A preliminary report
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DOI：
10.1136/ard.57.12.738
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发表时间：
1998-12-01
影响因子：
27.4
通讯作者：
Muñoz, E
中科院分区：
文献类型：
--
作者：
Collantes, E;Blázquez, MV;Muñoz, E
NF-κ B/Rel转录因子家族在转录水平调节参与免疫或炎症反应的许多基因的表达。本研究的目的是确定不同形式的关节疾病中是否存在NF-κ B活化的独特模式。方法在各种慢性风湿性疾病患者的纯化滑膜和外周T细胞中检测这些核蛋白的DNA结合活性（12例：类风湿性关节炎4例;脊柱关节病5例;结果-电泳迁移率变动分析揭示了两种特异性复合物与NF-κ B特异性P-32结合，从类风湿性关节炎患者的滑液和外周血分离的纯化T细胞提取的核蛋白中的标记寡核苷酸。复合物由p50/p50同源二聚体和p50/p65异源二聚体组成。相对于外周T细胞，观察到滑膜T细胞中NF-κ B与DNA结合增加。在非类风湿性关节炎，结合NF-κ B B在滑膜T细胞是专门介导的p50/p50 homodimeres.Conclusion-Overall，结果表明，NF-κ B B可能发挥了核心作用，在慢性类风湿性关节炎浸润T细胞的激活。这种核因子的激活在类风湿性滑膜T细胞中与其他形式的非类风湿性关节炎（例如骨关节炎、脊柱关节病）中有质的不同。
Objective-The NF-kappa B/Rel family of transcription factors regulates the expression of many genes involved in the immune or inflammatory response at the transcriptional level. The aim of this study was to determine whether distinctive patterns of NF-kappa B activation are seen in different forms of joint disease.Methods-The DNA binding activity of these nucleoproteins was examined in purified synovial and peripheral T cells from patients with various chronic rheumatic diseases (12: four with rheumatoid arthritis; five with spondyloarthropathies; and three with osteoarthritis).Results-Electrophoretic mobility shift assays disclosed two specific complexes bound to a NF-kappa B specific P-32-labelled oligonucleotide in nucleoproteins extracted from purified T cells isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis. The complexes consisted of p50/p50 homodimers and p50/p65 heterodimers. Increased NF-kappa B binding to DNA in synovial T cells was observed relative to peripheral T cells. In non-rheumatoid arthritis, binding of NF-kappa B in synovial T cells was exclusively mediated by p50/p50 homodimers.Conclusion-Overall, the results suggest that NF-kappa B may play a central part in the activation of infiltrating T cells in chronic rheumatoid arthritis. The activation of this nuclear factor is qualitatively different in rheumatoid synovial T cells to that in other forms of non-rheumatoid arthritis (for example, osteoarthritis, spondyloarthropathies).