A comparison of breast cancer tumor cells with varying expression of the Her2/neu receptor by Raman microspectroscopic imaging
A comparison of breast cancer tumor cells with varying expression of the Her2/neu receptor by Raman microspectroscopic imaging
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DOI：
10.1039/c0an00524j
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Otto, Cees
中科院分区：
文献类型：
--
作者：
Hartsuiker, Liesbeth;Zeijen, Nicole J. L.;Otto, Cees
Her 2/neu原癌基因在25%至30%的人类原发性乳腺癌中扩增。Her 2/neu的作用在文献中已有报道，显示出与细胞内脂质组成的不同关系。本研究利用拉曼显微光谱成像技术对不同程度表达Her 2/neu受体的乳腺癌细胞株MDA-MB-231、MDA-MB-435 s和SK-BR-3的单个活细胞的化学组成进行了研究。不同细胞群的平均拉曼光谱显示在所有细胞系中存在显著的脂质。拉曼差光谱显示MDA-MB细胞系中脂质含量增加，以及相对于SK-BR-3细胞的脂肪酸饱和度较低，具有高度显著性。这些结果证实了单细胞的层次聚类分析。通过对由来自单个细胞系的不同细胞的数据组成的单个矩阵进行分层聚类分析，显示了细胞系中化学组成的高度内部一致性。尽管SK-BR-3细胞的Her 2/neu表达最高，但它们的脂质含量低于表达较少或不表达Her 2/neu受体的MDA-MB细胞系。而不是代谢率或衰老，细胞的转移程度似乎与细胞的多不饱和脂肪酸含量有关。
The Her2/neu proto-oncogene is amplified in 25 to 30 percent of human primary breast carcinomas. The roles of Her2/neu have been reported before in literature, showing different relations to intracellular lipid composition. Here, we use Raman microspectroscopic imaging to reveal the chemical composition of single live cells from breast carcinoma cell lines MDA-MB-231, MDA-MB-435s and SK-BR-3, which express Her2/neu receptor in different extent. Average Raman spectra of the different cell populations show prominent lipid presence in all cell lines. With high significance, Raman difference spectra reveal increased lipid contents, as well as a lower degree of fatty acid saturation in the MDA-MB cell lines with respect to the SK-BR-3 cells. These results are confirmed by hierarchical cluster analysis of single cells. High internal consistency of the chemical compositions in the cell lines is shown by hierarchical cluster analysis on a single matrix composed of the data of different cells from a single cell line. Although Her2/neu expression is highest for SK-BR-3 cells, their lipid contents are lower than that of the MDA-MB cell lines, which express less to no Her2/neu receptors. Rather than metabolic rate or senescence, the degree of metastaticity of the cells appears to be related to the polyunsaturated fatty acid contents of the cells.