Macroporous chitin affinity membranes for lysozyme separation.
Macroporous chitin affinity membranes for lysozyme separation.
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DOI：
10.1002/(sici)1097-0290(19971220)56:6
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发表时间：
1997-12
影响因子：
3.8
通讯作者：
E. Ruckenstein;X. Zeng
中科院分区：
文献类型：
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作者：
E. Ruckenstein;X. Zeng
利用本实验室开发的以二氧化硅颗粒为成孔剂的技术，制备了具有高孔隙率、可控孔隙率和良好力学性能的大孔甲壳素膜。它们被用于溶菌酶的亲和分离。几丁质膜（1毫米厚）可以在高通量（>/=1.1 mL/min/cm(2)）下操作，对应于压降>/=2 psi。它们对溶菌酶的吸附能力（约50 mg/mL膜）比柱分离中使用的几丁质珠高一个数量级。在溶菌酶和卵清蛋白的二元混合物中，膜对溶菌酶有很高的选择性。考察了加载和洗脱过程中流速等重要操作参数的影响。从溶菌酶和卵清蛋白的混合物以及蛋清中获得了纯度极高的溶菌酶（>98%）。结果表明，大孔甲壳素膜可用于溶菌酶的大规模分离、纯化和回收。(c) 1997 John Wiley & Sons, Inc。中国生物医学工程学报（英文版），2004,31(4):444 - 444。
Macroporous chitin membranes with high, controlled porosity and good mechanical properties have been prepared using a technique developed in this laboratory based on silica particles as porogen. They were employed for the affinity separation of lysozyme. Chitin membranes (1 mm thickness) can be operated at high fluxes (>/=1.1 mL/min/cm(2)) corresponding to pressure drops >/=2 psi. Their adsorption capacity for lysozyme ( approximately 50 mg/mL membrane) is by an order of magnitude higher than that of the chitin beads employed in column separation. In a binary mixture of lysozyme and ovalbumin, the membranes showed very high selectivity towards lysozyme. The effect of some important operation parameters, such as the flow rates during loading and elution were investigated. Lysozyme of very high purity (>98%) was obtained from a mixture of lysozyme and ovalbumin, and from egg white. The results indicate that the macroporous chitin membranes can be used for the separation, purification, and recovery of lysozyme at large scale. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 610-617, 1997.