Herbicide atrazine exposure induce oxidative stress, immune dysfunction and WSSV proliferation in red swamp crayfish Procambarus clarkii.
Herbicide atrazine exposure induce oxidative stress, immune dysfunction and WSSV proliferation in red swamp crayfish Procambarus clarkii.
复制标题
除草剂莠去津暴露会导致红沼泽克氏原螯虾氧化应激、免疫功能障碍和 WSSV 增殖。
DOI：
10.1016/j.chemosphere.2021.131227
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发表时间：
2021-06
期刊：
影响因子：
8.8
通讯作者：
Wenzhi Wei
中科院分区：
文献类型：
--
作者：
Hui Yang;Yinan Jiang;Kaiyuan Lu;Haoran Xiong;Yingying Zhang;Wenzhi Wei
阿特拉津是一种潜在的环境内分泌干扰物，对动物具有多种毒性作用。它对水生生态系统有很大影响，但对其对甲壳动物免疫毒性的研究却很少。本研究以克氏原螯虾（Procambarus clarkii）为实验材料，研究了0.5 mg/L和5 mg/L阿特拉津暴露对克氏原螯虾的免疫毒性。在整个实验过程中，在5 mg/L阿特拉津暴露下观察到总血细胞计数（THC）显著降低。超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶活性受到明显抑制，活性氧（ROS）和丙二醛（MDA）含量升高，表现出潜在的氧化胁迫。综合生物标志物响应（IBR）的分析显示氧化应激生物标志物的诱导和抗氧化剂的抑制。5 mg/L阿特拉津染毒144 h后，克氏原螯虾肝胰腺的完整性受到破坏，肝小管间连接消失，肝小管空泡增多。测定了阿特拉津暴露后肝胰腺中不同免疫基因的相对表达水平。这些基因中的大多数被抑制，并表现出一定的剂量依赖性效应。结果表明，在5 mg/L浓度下，小龙虾白色斑点综合征病毒（WSSV）在肌肉中的含量显著高于其它浓度组，且死亡率显著高于其它浓度组。本研究确定了阿特拉津暴露对对虾白斑病爆发的影响，也为进一步评估农药对对虾白斑病的发生提供了重要依据。clarkii。
Atrazine is considered as a potential environmental endocrine disruptors and exhibits various toxic effects on animals. It has a great impact in the aquatic ecosystems, but there are few studies on its immunotoxicity in crustaceans. In the present study, theProcambarus clarkiiwere utilized to assess the immune toxicity after 0.5 mg/L and 5 mg/L atrazine exposure. A significant decrease in total hemocytes count (THC) was observed at 5 mg/L atrazine exposure throughout the experiment. The activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly inhibited, but the content of reactive oxygen species (ROS) and malondialdehyde (MDA) were up-regulated, indicating the potential oxidative stress. The analysis of the integrated biomarker response (IBR) showed the induction of oxidative stress biomarkers and the inhibition of antioxidants. After 5 mg/L atrazine exposure for 144 h, the integrity of crayfish hepatopancreas was destroyed with disappeared connections between tubules and increased liver tubules vacuoles. The relative expression levels of different immune genes in hepatopancreas after atrazine exposure were measured. Most of these genes were suppressed and exhibited a certain dose-dependent effect. The results of crayfish white spot syndrome virus (WSSV) replication shown the amount of virus in muscle was significantly higher and exhibited a higher mortality rate at 5 mg/L group than other groups. The present study determined the impact of atrazine exposure on WSSV outbreaks, and also provide an important basis for further assessing the occurrence of pesticides on diseases ofP. clarkii.