In vivo anti-arthritic efficacy of Camellia sinensis (L.) in collagen induced arthritis model.
In vivo anti-arthritic efficacy of Camellia sinensis (L.) in collagen induced arthritis model.
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DOI：
10.1016/j.biopha.2016.12.089
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发表时间：
2017-03
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通讯作者：
A. Tanwar;R. Chawla;Md Meraj Ansari;Neha;P. Thakur;A. Chakotiya;R. Goel;H. Ojha;M. Asif;M. Basu;R. Arora;H. Khan
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作者：
A. Tanwar;R. Chawla;Md Meraj Ansari;Neha;P. Thakur;A. Chakotiya;R. Goel;H. Ojha;M. Asif;M. Basu;R. Arora;H. Khan
研究背景风湿性关节炎（Rheumatoid arthritis，RA）是一种自身免疫性炎症性疾病，伴有滑膜增生、软骨破坏、骨破坏等，常伴有感染的危险。这种风险可归因于免疫抑制剂的使用。因此，本研究进行评估的水-醇提取物的茶树（L.）的抗关节炎的疗效。材料和方法茶树（Camellia sinensis（L.）或Cs过滤并通过索氏装置在1：1水溶液：乙醇中萃取，然后冻干和喷雾干燥以形成无定形粉末。四种不同的口服剂量（50、100、200、400 mg/kg/体重）在胶原诱导的关节炎模型中评价水醇提取物的抗水肿作用。针对氧化应激标志物如谷氨酸[5- 5 '二硫代-双-2-硝基苯甲酸（DTNB）]、超氧化物歧化酶[肾上腺素]、过氧化氢酶[过氧化氢]、脂质过氧化[硫代巴比妥酸反应物质（TBARS）]、一氧化氮[Griess试剂：关节中的关节弹性蛋白酶[N-甲氧基琥珀酰-Ala-Ala-Pro-Val-硝基苯胺]，随后进行血液学评价，包括RBC、WBC、血红蛋白，血小板和红细胞压积为了验证这些生化变化，放射学和组织病理学（苏木精和伊红）evaluations.ResultsThe选定的抗关节炎剂量的Cs，即400 mg/kg/体重。（在第35天约60%的抗关节炎功效）可归因于酶（超氧化物歧化酶和过氧化氢酶）和非酶（谷氨酸）抗氧化剂的水平分别显著（p < 0.05）增加34%、59%和50%。同时，与对照组相比，脂质过氧化物、亚硝酸盐自由基和弹性蛋白酶活性分别显着（p<0.05）降低32%、45%和32%，表明氧化应激总体下降。血液学评价显示治疗组的红细胞、白细胞和血小板水平恢复。利用放射学和组织学评估的验证性分析显示，与胶原蛋白诱导的关节炎相比，治疗组的关节畸形、组织肿胀、血管翳形成和中性粒细胞浸润均有所缓解。结论分析表明，Cs可以通过调节抗氧化剂水平在减少氧化应激方面发挥有效作用，降低自由基水平，同时恢复正常的造血级联，如在胶原诱导的关节炎模型中所观察到的。因此，400 mg/kg体重的累积剂量影响，在14天的时间内，也发现在保护它们的结构一致性以对抗这种自身免疫疾病方面极其有效。
BackgroundRheumatoid arthritis (RA), an autoimmune inflammatory disorder with synovial hyperplasia, destruction of cartilage, bone damage is often associated with risk of infections. Such risk could be attributed towards usage of immunosuppressive agents. Thus, the present study was undertaken to evaluate the anti-arthritic efficacy of aquo-alcoholic extract ofCamellia sinensis(L.).Material and methodsDried leaves ofCamellia sinensis(L.) or Cs were filtered and extracted in 1:1 aqueous: ethanol by Soxhlet apparatus followed by lyophilization and spray drying to develop amorphous powder. Four different oral doses (50, 100, 200, 400mg/kg/body wt.) of aquo-alcoholic extract were evaluated for anti-edematogenic effect in collagen induced arthritis model. The selected anti-arthritic doses of Cs were evaluated for the oxidative stress markers like Glutathione [5-5’dithio-bis-2-nitrobenzoicacid (DTNB)], Superoxide dismutase [Epinephrine], Catalase [Hydrogen peroxide], Lipid peroxidation [Thiobarbituric acid reactive substance (TBARS)], Nitric oxide [Griess reagents:Nitrobluetetrazolium], Articular elastase [N-methoxysuccinyl-Ala-Ala-Pro- Valp-nitroanilide] in joints followed by haematological evaluation including RBC, WBC, Haemoglobin, platelets and haematocrit. To validate these biochemical changes, the radiological and histopathological (Haematoxylin & Eosin) evaluation was also conducted.ResultsThe selected anti-arthritic dose of Cs i.e.400mg/kg/body wt. (∼60% anti-arthritic efficacy on 35th day) could be attributed towards significant (p < 0.05) increase in the levels of enzymatic (Superoxide dismutase and Catalase) and non-enzymatic (Glutathione) antioxidants by 34%, 59% and 50% respectively. Simultaneously, the significant(p<0.05)reduction of lipid peroxides, nitrite radical and elastase activity by 32%, 45% & 32% respectively as compare to control indicated overall decrease in oxidative stress. Haematological evaluation revealed restoration of RBC, WBC and platelets level in treatment group. The confirmatory analysis utilizing radiological and histological assessment showed alleviation of joint deformity, tissue swelling, pannus formation and neutrophils infiltration in treatment group as compared to collagen induced arthritis.ConclusionThe analysis showed that Cs can play an effective role in reduction of oxidative stress by modulating levels of antioxidants, reducing levels of free radicals while restoring normal haematopoietic cascade as observed in collagen induced arthritis model. Thus, the cumulative dose impact of400mg/kg body wt., over a period of 14 days also found extremely effective in terms of safeguarding their structural conformity against such auto-immune disorder.