Carbamylated low-density lipoprotein induces oxidative stress and accelerated senescence in human endothelial progenitor cells
Carbamylated low-density lipoprotein induces oxidative stress and accelerated senescence in human endothelial progenitor cells
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DOI：
10.1096/fj.10-173377
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发表时间：
2011-04-01
期刊：
影响因子：
4.8
通讯作者：
Ramirez, Rafael
中科院分区：
文献类型：
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作者：
Carracedo, Julia;Merino, Ana;Ramirez, Rafael
氨甲酰化低密度脂蛋白（cLDL）在动脉粥样硬化中起作用。在这项研究中，我们评估了尿毒症对LDL氨甲酰化的影响，以及cLDL和氧化LDL （oxLDL; 200 μ g/ml）对健康志愿者内皮祖细胞（EPCs）数量、功能和基因组稳定性的影响。cLDL是在慢性肾脏疾病（CKD） 2-4期患者的天然LDL （nLDL）与尿毒症血清孵育后产生的。流式细胞术和荧光显微镜检测氧化应激，流式细胞术检测线粒体去极化，β -半乳糖苷酶活性和端粒长度检测衰老，磷酸化组蛋白H2AX （γ H2AX）检测DNA损伤。尿毒症血清cLDL百分比与CKD严重程度相关。与nLDL相比，cLDL诱导氧化应激增加(62 +/- 5比8 +/- 3%，P
Carbamylated low-density lipoprotein (cLDL) plays a role in atherosclerosis. In this study we evaluate the effect of uremia on LDL carbamylation and the effect of cLDL and oxidized LDL (oxLDL; 200 mu g/ml) on number, function, and genomic stability of endothelial progenitor cells (EPCs) obtained from healthy volunteers. cLDL was generated after incubation of native LDL (nLDL) with uremic serum from patients with chronic kidney disease (CKD) stages 2-4. Oxidative stress was measured by flow cytometry and fluorescent microscopy, mitochondrial depolarization by flow cytometry, senescence by beta-galactosidase activity and telomere length, and DNA damage by phosphorylated histone H2AX (gamma H2AX). The percentage of cLDL by uremic serum was related to the severity of CKD. Compared with nLDL, cLDL induced an increase in oxidative stress (62 +/- 5 vs. 8 +/- 3%, P