Impact of Pseudomonas aeruginosa Genomic Instability on the Application of Typing Methods for Chronic Cystic Fibrosis Infections
Impact of Pseudomonas aeruginosa Genomic Instability on the Application of Typing Methods for Chronic Cystic Fibrosis Infections
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DOI：
10.1128/jcm.00019-10
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发表时间：
2010-06-01
影响因子：
9.4
通讯作者：
Winstanley, Craig
中科院分区：
文献类型：
--
作者：
Fothergill, Joanne L.;White, Judith;Winstanley, Craig
铜绿假单胞菌的利物浦流行株（LES）在英国囊性纤维化（CF）患者中广泛传播，最近在北美出现。在这项研究中，我们报告了24个“异常”的CF分离的铜绿假单胞菌，产生不一致的结果，无论是脉冲场凝胶电泳（PFGE）或PCR测试的LES分析。我们使用了一种新的分型方法，ArrayTube基因分型系统，以确定24个异常分离株测试，13个被确认为LES。LES菌株不能清楚地区分从非LES菌株的其他两个常用的遗传指纹图谱测试，随机扩增多态性DNA（RAPD）分析和BOX-PCR，并在他们的运载LES基因组岛和原噬菌体的变化很大。LES的基因组不稳定性表明，鉴定这种新出现的传染性菌株可能是一项具有挑战性的任务，并且它质疑在慢性CF感染的背景下，区分是否总是细菌分型方法的理想特征。
The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients in the United Kingdom and has emerged recently in North America. In this study, we report the analysis of 24 "anomalous" CF isolates of P. aeruginosa that produced inconsistent results with regard to either pulsed-field gel electrophoresis (PFGE) or PCR tests for the LES. We used a new typing method, the ArrayTube genotyping system, to determine that of the 24 anomalous isolates tested, 13 were confirmed as the LES. LES isolates could not be clearly distinguished from non-LES isolates by two other commonly used genetic fingerprinting tests, randomly amplified polymorphic DNA (RAPD) analysis and BOX-PCR, and varied considerably in their carriage of LES genomic islands and prophages. The genomic instability of the LES suggests that identification of this emerging transmissible strain could be a challenging task, and it questions whether discrimination is always a desirable feature of bacterial typing methods in the context of chronic CF infections.