EFFECTS OF ALLOPURINOL [4-HYDROXYPYRAZOLO(3,4-D)PYRIMIDINE] ON METABOLISM OF ALLANTOIN IN SOYBEAN PLANTS
EFFECTS OF ALLOPURINOL [4-HYDROXYPYRAZOLO(3,4-D)PYRIMIDINE] ON METABOLISM OF ALLANTOIN IN SOYBEAN PLANTS
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DOI：
10.1104/pp.62.1.134
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发表时间：
1978-01-01
期刊：
影响因子：
7.4
通讯作者：
YAMAGUCHI, M
中科院分区：
文献类型：
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作者：
FUJIHARA, S;YAMAGUCHI, M
研究了黄嘌呤氧化酶抑制剂别嘌醇[4-羟基吡唑(3，4-d)嘧啶]对大豆植株尿囊素代谢的影响。Tamanishiki)被报道。大豆幼苗在含有1 mM别嘌醇的琼脂上无菌萌发96h，与对照相比，只含有少量的尿囊素、尿囊酸和尿素。对别嘌醇处理的幼苗的嘌呤和嘧啶的分析表明，黄嘌呤在子叶和苗轴中都有明显的积累。未发现次黄嘌呤的积累。子叶落叶后，别嘌醇处理引起的黄嘌呤积累相对较低。对于结瘤植物[Rhizobriuns Japan onicum]，别嘌醇引起茎和根瘤中尿囊素(+尿囊酸)的显著下降，并伴随着黄嘌呤在根瘤中的显著积累。黄嘌呤在根瘤中的浓度远高于萌发幼苗。50微米的别嘌醇对大豆根瘤中的黄嘌呤氧化酶有较强的抑制作用。结果表明，大豆体内尿囊素形成的主要途径是通过嘌呤分解，通过黄嘌呤尿酸。特别指出，大豆根瘤中存在一个非常活跃的嘌呤分解系统。
Some studies on the effects of xanthine oxidase inhibitor allopurinol [4-hydroxypyrazolo(3,4-d)pyrimidine] on allantoin metabolism of soybean plants (Glycine max cv. Tamanishiki) are reported. Soybean seedlings, aseptically germinated for 96 h on agar containing 1 mM allopurinol, contained only slight amounts of allantoin, allantoic acid and urea as compared with controls. Analysis of purines and pyrimidines of the allopurinol-treated seedlings showed marked accumulation of xanthine both in the cotyledons and seedling axes. No hypoxanthine accumulation was found. Xanthine accumulation due to allopurinol treatment was relatively low after the cotyledons had fallen. For nodulated plants [Rhizobriuns japonicum], allopurinol caused a significant drop in allantoin (+allantoic acid) in the stems and nodules, accompanied by a striking accumulation of xanthine in the nodules. The xanthine concentration in the nodules far exceeded that in the germinated seedlings. Allopurinol at a concentration of 50 .mu.M strongly inhibited xanthine oxidase prepared from soybean nodules. The results suggested that the main pathway of allantoin formation in soybean plants was through purine decomposition, via xanthine-uric acid. It was specially noted that a very active purine-decomposing system existed in soybean nodules.