Chaperonin (HSP60) and annexin-2 are candidate biomarkers for non-small cell lung carcinoma.
Chaperonin (HSP60) and annexin-2 are candidate biomarkers for non-small cell lung carcinoma.
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DOI：
10.1097/md.0000000000005903
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发表时间：
2017-02
期刊：
影响因子：
1.6
通讯作者：
Akkoçlu A
中科院分区：
文献类型：
--
作者：
Ağababaoğlu İ;Önen A;Demir AB;Aktaş S;Altun Z;Ersöz H;Şanl A;Özdemir N;Akkoçlu A
肺癌分别占所有新诊断癌症病例和癌症死亡率的12.4%和17.6%，尽管所有改进的治疗方案，5年生存率为15%。在一期和手术治疗的患者中，生存率达到66%。早期诊断对提高本病的生存率至关重要，而早期诊断尚不能明确和普遍地实现。由于这个原因；采用基质辅助激光解吸电离（MALDI）质谱法评价肺腺癌和鳞状细胞癌亚组的蛋白质组学差异。36例恶性肿瘤患者的新鲜组织样本，男性占83.3% (n = 30)，女性占16.7% (n = 6)，分为早期和终末期肺癌2组，每组包括18例鳞状细胞癌（9例早期，9例终末期）和18例腺癌（9例早期，9例终末期）。手术干预后从肿瘤和匹配的正常部位获得的新鲜组织。通过比较每个患者的蛋白质组学变化来确定蛋白质表达水平的差异。在晚期腺癌亚组中；肿瘤组织与正常实质组织相比，2种蛋白的表达存在差异。这些;热休克蛋白60 （HSP60）蛋白表达差异有统计学意义（P = 0.0001）。早期和晚期鳞状细胞癌亚群在正常组织和病变鳞状细胞癌的某些20蛋白表达上存在差异。其中，仅有annexin-2蛋白表达水平升高，差异有统计学意义（P = 0.002）。腺癌和鳞状细胞癌亚组早期和晚期肿瘤组织蛋白表达无显著差异。我们得出结论，在肺癌的早期诊断方面，HSP60和annexin-2蛋白是腺癌和鳞状细胞癌亚组中重要的生物标志物。我们还认为，这两种蛋白可能在评估预后、转移潜力、治疗反应以及建立腺癌和鳞状细胞癌的鉴别诊断方面发挥重要作用。
Lung cancer is responsible of 12.4% and 17.6% of all newly diagnosed cancer cases and mortality due to cancer, respectively, and 5-year survival rate despite all improved treatment options is 15%. This survival rate reaches 66% in the Stage 1 and surgically treated patients. Early diagnosis which could not be definitely and commonly achieved yet is extremely critical in obtaining high survival rate in this disease. For this reason; proteomic differences were evaluated using matrix assisted laser desorption ionization (MALDI) mass spectrometry in the subgroups of lung adenocarcinoma and squamous cell carcinoma. Fresh tissue samples of 36 malignant cases involving 83.3% (n = 30) men and 16.7% (n = 6) women patients were distributed into 2 groups as early and end stage lung cancer and each group were composed of subgroups including 18 squamous cell carcinoma (9 early stage cases, 9 end stage cases) and 18 adenocarcinoma cases (9 early stage cases, 9 end stage cases). The fresh tissues obtained from the tumoral and matched normal sites after surgical intervention. The differences in protein expression levels were determined by comparing proteomic changes in each patient. In the subgroups of advanced stage adenocarcinoma; tumoral tissue revealed differences in expression of 2 proteins compared with normal parenchymal tissue. Of those; difference in protein expression in heat shock protein 60 (HSP60) was found statistically significant (P = 0.0001). Subgroups of early and advanced stage squamos cell carcinoma have differed in certain 20 protein expression of normal tissue and diseased squamos cell carcinoma. Of those, increased protein expression level of only annexin-2 protein was found statistically significant (P = 0.002). No significant difference was detected in early and advanced stage protein expressions of the tumoral tissues in the subgroups of adenocarcinoma and squamous cell carcinoma. We conclude that with respect to early diagnosis of lung cancer that HSP60 and annexin-2 proteins are the important biomarkers in the subgroups of adenocarcinoma and squamous cell carcinoma. We also consider that these 2 proteins are molecules which may provide critical contribution in evaluation of prognosis, metastatic potential, response to treatment, and in establishment of differential diagnosis between adenocarcinoma and squamous cell carcinoma.